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Optimizing UPLC isocyanate determination through a Taguchi experimental design approach

2013-05Artigo científico Acesso restrito
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dc.contributor.authorAndré, Catarina
dc.contributor.authorJorge, Fabiana
dc.contributor.authorCastanheira, Isabel
dc.contributor.authorMatos, Ana
dc.date.accessioned2014-01-31T14:02:48Z
dc.date.available2014-01-31T14:02:48Z
dc.date.issued2013-05
dc.description.abstractThe development of a chromatographic procedure for an ultra-performance liquid chromatography can be a very time-consuming task, as the general approach for finding the appropriate operating conditions has been a trialand- error process. The present study reports a novel approach in the field of ultra-performance liquid chromatography by using statistical experimental design based on Taguchi’s method, which allows a complete separation of nine isocyanates present in a complex matrix. The resolution between two adjacent peaks was considered as a quality characteristic and transformed to a Taguchi signal-to-noise ratio. An orthogonal array L9 (34) was selected to analyze the effect of four chromatographic factors, that is, proportion of solvent, percent triethylamine (v/v), temperature ( C), and flow (mLmin 1), with three levels each. The joint analysis performed to the significant factors achieved in the eight analyses of variance allowed to identify two methods to conduct a complete separation of all peaks. Six isocyanates were separated with the first method, with all factors at the lowest level. The remaining three isocyanates were separated with the second method, with the proportion of solvent at the highest level and the other factors at the lowest level. The overall Taguchi experimental design identified the proportion of solvent and the flow rate as major chromatographic factors. Finally, confirmatory experiments were performed with samples prepared with six and three isocyanates, confirming the complete separation of all isocyanates in the study. The Taguchi methods provided a systematic and efficient methodology for this optimization, with considerably less effort than would be required for other optimizations techniques.por
dc.description.sponsorshipPortuguese Innovation Agency (ADI) in the frame of project QREN 5012-LIRACorkpor
dc.identifier.citationJ Chemometrics. 2013; 27: 91–98por
dc.identifier.issn0886-9383
dc.identifier.otherdoi:10.1002/cem.2496
dc.identifier.urihttp://hdl.handle.net/10400.18/1898
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherJohn Wiley and Sonspor
dc.relationQREN 5012-LIRACork.por
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/cem.2496/fullpor
dc.subjectFood Safetypor
dc.subjectTaguchi Experimental Designpor
dc.subjectIsocyanatespor
dc.subjectUPLCpor
dc.subjectSignal-to-noise Ratiopor
dc.subjectUltra-performance Liquid Chromatographypor
dc.subjectSegurança Alimentarpor
dc.titleOptimizing UPLC isocyanate determination through a Taguchi experimental design approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage98por
oaire.citation.startPage91por
oaire.citation.titleJournal of Chemometricspor
oaire.citation.volume27por
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

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