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Optimizing UPLC isocyanate determination through a Taguchi experimental design approach
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Orientador(es)
Resumo(s)
The development of a chromatographic procedure for an ultra-performance liquid chromatography can be a very
time-consuming task, as the general approach for finding the appropriate operating conditions has been a trialand-
error process. The present study reports a novel approach in the field of ultra-performance liquid
chromatography by using statistical experimental design based on Taguchi’s method, which allows a complete
separation of nine isocyanates present in a complex matrix. The resolution between two adjacent peaks was
considered as a quality characteristic and transformed to a Taguchi signal-to-noise ratio. An orthogonal array
L9 (34) was selected to analyze the effect of four chromatographic factors, that is, proportion of solvent, percent
triethylamine (v/v), temperature ( C), and flow (mLmin 1), with three levels each. The joint analysis performed
to the significant factors achieved in the eight analyses of variance allowed to identify two methods to conduct
a complete separation of all peaks. Six isocyanates were separated with the first method, with all factors at the
lowest level. The remaining three isocyanates were separated with the second method, with the proportion of
solvent at the highest level and the other factors at the lowest level. The overall Taguchi experimental design
identified the proportion of solvent and the flow rate as major chromatographic factors. Finally, confirmatory
experiments were performed with samples prepared with six and three isocyanates, confirming the complete
separation of all isocyanates in the study.
The Taguchi methods provided a systematic and efficient methodology for this optimization, with considerably less
effort than would be required for other optimizations techniques.
Descrição
Palavras-chave
Food Safety Taguchi Experimental Design Isocyanates UPLC Signal-to-noise Ratio Ultra-performance Liquid Chromatography Segurança Alimentar
Contexto Educativo
Citação
J Chemometrics. 2013; 27: 91–98