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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

2020-12Journal article Open access
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dc.contributor.authorMøller, Peter
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorBoutet-Robinet, Elisa
dc.contributor.authorKoppen, Gudrun
dc.contributor.authorBonassi, Stefano
dc.contributor.authorMilić, Mirta
dc.contributor.authorGajski, Goran
dc.contributor.authorCosta, Solange
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta Pereira, Cristiana
dc.contributor.authorDusinska, Maria
dc.contributor.authorGodschalk, Roger
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorGutzkow, Kristine B.
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorCooke, Marcus S.
dc.contributor.authorRichling, Elke
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorBasaran, Nursen
dc.contributor.authorDel Bo’, Cristian
dc.contributor.authorZegura, Bojana
dc.contributor.authorNovak, Matjaz
dc.contributor.authorStopper, Helga
dc.contributor.authorVodicka, Pavel
dc.contributor.authorVodenkova, Sona
dc.contributor.authorde Andrade, Vanessa Moraes
dc.contributor.authorSramkova, Monika
dc.contributor.authorGabelova, Alena
dc.contributor.authorCollins, Andrew
dc.contributor.authorLangie, Sabine A.S.
dc.date.accessioned2021-03-03T16:34:36Z
dc.date.available2021-03-03T16:34:36Z
dc.date.issued2020-12
dc.descriptionReviewpt_PT
dc.description.abstractThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNat Protoc. 2020 Dec;15(12):3817-3826. doi: 10.1038/s41596-020-0398-1. Epub 2020 Oct 26.pt_PT
dc.identifier.doi10.1038/s41596-020-0398-1pt_PT
dc.identifier.issn1754-2189
dc.identifier.urihttp://hdl.handle.net/10400.18/7298
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relation.publisherversionhttps://www.nature.com/articles/s41596-020-0398-1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDNA Damagept_PT
dc.subjectComet Assaypt_PT
dc.subjectMinimum Information for Reporting Comet Assay (MIRCA)pt_PT
dc.subjectRecommendationspt_PT
dc.titleMinimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and resultspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3826pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage3817pt_PT
oaire.citation.titleNature Protocolspt_PT
oaire.citation.volume15pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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