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Subproteomic signature comparison of in vitro selected fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104B

2017-10Journal article Restricted access
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dc.contributor.authorCorreia, Susana
dc.contributor.authorHébraud, Michel
dc.contributor.authorChafsey, Ingrid
dc.contributor.authorChambon, Christophe
dc.contributor.authorViala, Didier
dc.contributor.authorTorres, Carmen
dc.contributor.authorCaniça, Manuela
dc.contributor.authorCapelo, José Luis
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.date.accessioned2018-03-07T18:49:38Z
dc.date.available2018-03-07T18:49:38Z
dc.date.issued2017-10
dc.description.abstractBackground: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strain (Se6), and also in Se6-M under ciprofloxacin (CIP) stress, were evaluated in order to give new insights into the mechanisms involved. Proteomes were compared at the intracellular and membrane levels by a 2-DE~LC-MS/MS and a shotgun LC-MS/MS approach, respectively. Results: In total, 35 differentially abundant proteins were identified when comparing Se6 with Se6-M (25 more abundant in Se6 and 10 more abundant in Se6-M) and 82 were identified between Se6-M and Se6-M+CIP (51 more abundant in Se6-M and 31 more abundant under ciprofloxacin stress). Conclusion: Several proteins with known and possible roles in quinolone resistance were identified which provide important information about mechanism-related differential protein expression, supporting the current knowledge and also leading to new testable hypotheses on the mechanism of action of fluoroquinolone drugs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationExpert Rev Proteomics. 2017 Oct;14(10):941-961. doi: 10.1080/14789450.2017.1375856. Epub 2017 Sep 18.pt_PT
dc.identifier.doi10.1080/14789450.2017.1375856pt_PT
dc.identifier.issn1478-9450
dc.identifier.urihttp://hdl.handle.net/10400.18/5355
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttp://www.tandfonline.com/doi/full/10.1080/14789450.2017.1375856pt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectComparative Subproteomicspt_PT
dc.subjectLC-MS/MSpt_PT
dc.subjectSalmonella Typhimurium DT104Bpt_PT
dc.subjectShotgun Proteomicspt_PT
dc.subject2-DEpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleSubproteomic signature comparison of in vitro selected fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104Bpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage961pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage941pt_PT
oaire.citation.titleExpert Review of Proteomicspt_PT
oaire.citation.volume14pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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