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Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

2023-08-17Journal article Open access
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dc.contributor.authorNunes, Alexandra
dc.contributor.authorOleastro, Mónica
dc.contributor.authorAlves, Frederico
dc.contributor.authorLiassine, Nadia
dc.contributor.authorLowe, David M.
dc.contributor.authorBenejat, Lucie
dc.contributor.authorDucounau, Astrid
dc.contributor.authorJehanne, Quentin
dc.contributor.authorBorges, Vítor
dc.contributor.authorGomes, João Paulo
dc.contributor.authorGodbole, Gauri
dc.contributor.authorPhilippe, Lehours
dc.date.accessioned2024-01-12T16:23:51Z
dc.date.available2024-01-12T16:23:51Z
dc.date.issued2023-08-17
dc.descriptionErratum in: Microbiol Spectr. 2023 Dec 12;11(6):e0312123. doi: 10.1128/spectrum.03121-23. Epub 2023 Oct 10.pt_PT
dc.descriptionFree PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434052/pt_PT
dc.description.abstractWe present two independent cases of recurrent multidrug-resistant Campylobacter jejuni infection in immunocompromised hosts and the clinical challenges encountered due to the development of high-level carbapenem resistance. The mechanisms associated with this unusual resistance for Campylobacters were characterized. Initial macrolide and carbapenem-susceptible strains acquired resistance to erythromycin (MIC . 256mg/L), ertapenem (MIC . 32mg/L), and meropenem (MIC . 32mg/L) during treatment. Carbapenem-resistant isolates developed an in-frame insertion resulting in an extra Asp residue in the major outer membrane protein PorA, within the extracellular loop L3 that connects b-strands 5 and 6 and forms a constriction zone involved in Ca21 binding. The isolates presenting the highest MIC to ertapenem exhibited an extra nonsynonymous mutation (G167AjGly56Asp) at PorA’s extracellular loop L1. IMPORTANCE Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.pt_PT
dc.description.abstractImportance: Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.pt_PT
dc.description.sponsorshipThis work was partially supported by GenomePT (ref. POCI-01-0145-FEDER-022184) from Fundação para a Ciência e Tecnologia, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicrobiol Spectr. 2023 Aug 17;11(4):e0107023. doi: 10.1128/spectrum.01070-23. Epub 2023 Jun 26.pt_PT
dc.identifier.doi10.1128/spectrum.01070-23pt_PT
dc.identifier.issn2165-0497
dc.identifier.urihttp://hdl.handle.net/10400.18/8898
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNational Facility for genome sequencing and analysis
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/spectrum.01070-23pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCampylobacter jejunipt_PT
dc.subjectRecurrent Infectionpt_PT
dc.subjectHigh-level Carbapenem Resistancept_PT
dc.subjectimmunocompromised Patientspt_PT
dc.subjectMacrolide Resistancept_PT
dc.subjectMajor Porinpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleRecurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNational Facility for genome sequencing and analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22184%2F2016/PT
oaire.citation.issue4pt_PT
oaire.citation.startPagee0107023pt_PT
oaire.citation.titleMicrobiology Spectrumpt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream9444 - RNIIIE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication3b8a803c-37e5-4354-93f5-6410d7fb2af7
relation.isProjectOfPublication.latestForDiscovery3b8a803c-37e5-4354-93f5-6410d7fb2af7

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