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Publication
Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
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Advisor(s)
Abstract(s)
We present two independent cases of recurrent multidrug-resistant
Campylobacter jejuni infection in immunocompromised hosts and the clinical challenges
encountered due to the development of high-level carbapenem resistance. The mechanisms
associated with this unusual resistance for Campylobacters were characterized.
Initial macrolide and carbapenem-susceptible strains acquired resistance to erythromycin
(MIC . 256mg/L), ertapenem (MIC . 32mg/L), and meropenem (MIC . 32mg/L) during
treatment. Carbapenem-resistant isolates developed an in-frame insertion resulting in an
extra Asp residue in the major outer membrane protein PorA, within the extracellular
loop L3 that connects b-strands 5 and 6 and forms a constriction zone involved in Ca21
binding. The isolates presenting the highest MIC to ertapenem exhibited an extra nonsynonymous
mutation (G167AjGly56Asp) at PorA’s extracellular loop L1.
IMPORTANCE Carbapenem susceptibility patterns suggest drug impermeability, related
to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular
events occurring in two independent cases support the association of these
mechanisms with carbapenem resistance in Campylobacter spp.
Importance: Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.
Importance: Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.
Description
Erratum in: Microbiol Spectr. 2023 Dec 12;11(6):e0312123. doi: 10.1128/spectrum.03121-23. Epub 2023 Oct 10.
Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434052/
Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434052/
Keywords
Campylobacter jejuni Recurrent Infection High-level Carbapenem Resistance immunocompromised Patients Macrolide Resistance Major Porin Infecções Gastrointestinais
Pedagogical Context
Citation
Microbiol Spectr. 2023 Aug 17;11(4):e0107023. doi: 10.1128/spectrum.01070-23. Epub 2023 Jun 26.