Publication
Assessment of the Genotoxic Hazard of Estuarine Sediments Using an Integrative Approach With LacZ PlasmidāBased Transgenic Mice
| dc.contributor.author | Pinto, Miguel | |
| dc.contributor.author | Sacadura, Joana | |
| dc.contributor.author | Costa, Pedro M. | |
| dc.contributor.author | Caeiro, Sandra | |
| dc.contributor.author | Louro, Henriqueta | |
| dc.contributor.author | Silva, Maria J. | |
| dc.date.accessioned | 2025-11-13T11:05:25Z | |
| dc.date.available | 2025-11-13T11:05:25Z | |
| dc.date.issued | 2025-06-16 | |
| dc.description.abstract | Under the influence of multiple anthropogenic pressures, from industrial to agricultural activities, estuaries have long been regarded as particularly sensitive ecosystems to contamination. The present study aimed at investigating the genotoxic potential of a contaminated sediment sample from an urban and industrial area of the Sado Estuary, by combining the analysis of multiple endpoints in the <jats:italic>LacZ</jats:italic> plasmidābased transgenic mouse model exposed for 28ādays to contaminated estuarine sediment extracts through drinking water. The DNA and chromosome damaging effects were monitored in peripheral blood at 7āday intervals using the standard and enzymeāmodified Comet assay, as well as the micronucleus assays in peripheral blood cells. After euthanasia, DNA damage was analyzed in several mouse tissues, and <jats:italic>LacZ</jats:italic> mutant frequencies were determined in the liver. Livers were also surveyed for histopathological analysis. A timeādependent increase in micronuclei frequency was seen at all tested doses, in spite of no induction of DNA damage in any organ or mutation induction in the liver of exposed mice. The liver from mice exposed to sediment extracts did not reveal major alterations besides evidence of inflammation. Overall, the integration of the endpoints analyzed in the mice is suggestive of potential chronic, rather than acute, adverse effects in vivo, and points to the need for further research in the resident human population in the area. This experimental design can be used to assess the genotoxicity of complex environmental mixtures, understand how they work, and reduce costs and resources while speeding up data collection and interpretation. | eng |
| dc.description.sponsorship | The authors would like to thank Edwin Zwart (RVIM, Bilthoven, The Netherlands) for support with the LacZ mutation assay and Andrew R. Collins (Oslo University, Norway) for supplying the FPG and ENDO III enzymes. The present research was funded by the Portuguese Foundation for Science and Technology (FCT) and co-financed by the European Community FEDER through the program COMPETE (project reference PTDC/SAU-ESA/100107/2008). This work was also financed by national funds from FCT in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular BiosciencesāUCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomyāi4HB. | |
| dc.identifier.citation | Environ Mol Mutagen. 2025 Aug;66(6-7):300-310. doi: 10.1002/em.70019. Epub 2025 Jun 16 | |
| dc.identifier.doi | 10.1002/em.70019 | |
| dc.identifier.eissn | 1098-2280 | |
| dc.identifier.issn | 0893-6692 | |
| dc.identifier.pmid | 40521651 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10602 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Wiley | |
| dc.relation | PTDC/SAU-ESA/100107/2008 | |
| dc.relation | Applied Molecular Biosciences Unit | |
| dc.relation | Institute for Health and Bioeconomy | |
| dc.relation | Applied Molecular Biosciences Unit | |
| dc.relation.hasversion | https://onlinelibrary.wiley.com/doi/10.1002/em.70019 | |
| dc.relation.ispartof | Environmental and Molecular Mutagenesis | |
| dc.relation.ispartofseries | 66(6-7):300-310. | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | |
| dc.subject | Estuary Sediments | |
| dc.subject | Genotoxicity | |
| dc.subject | Histopathology | |
| dc.subject | LacZ Mice | |
| dc.subject | Mutant Frequency | |
| dc.subject | Genotoxicidade Ambiental | |
| dc.title | Assessment of the Genotoxic Hazard of Estuarine Sediments Using an Integrative Approach With LacZ PlasmidāBased Transgenic Mice | eng |
| dc.type | journal article | |
| dcterms.references | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fem.70019&file=em70019-sup-0001-TableS1.docx | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Applied Molecular Biosciences Unit | |
| oaire.awardTitle | Institute for Health and Bioeconomy | |
| oaire.awardTitle | Applied Molecular Biosciences Unit | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ESA%2F100107%2F2008/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT | |
| oaire.citation.endPage | 310 | |
| oaire.citation.issue | 6-7 | |
| oaire.citation.startPage | 300 | |
| oaire.citation.title | Environmental and Molecular Mutagenesis | |
| oaire.citation.volume | 66 | |
| oaire.fundingStream | 5876-PPCDTI | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Louro | |
| person.givenName | Henriqueta | |
| person.identifier | 157627 | |
| person.identifier.ciencia-id | 721D-2BB1-7DB1 | |
| person.identifier.orcid | 0000-0001-9744-7332 | |
| person.identifier.scopus-author-id | 6507971479 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia | |
| project.funder.name | FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia | |
| project.funder.name | FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia | |
| project.funder.name | FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia | |
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