Repository logo
 
Publication

The third dimension: new developments in cell culture models for colorectal research

2016-11Journal article Restricted access
Simple item page
dc.contributor.authorPereira, Joana
dc.contributor.authorAvatade, Nikhil
dc.contributor.authorLoureiro, Cláudia
dc.contributor.authorMatos, Paulo
dc.contributor.authorAmaral, Margarida
dc.contributor.authorJordan, Peter
dc.date.accessioned2016-10-03T12:10:03Z
dc.date.available2017-05-01T00:30:11Z
dc.date.issued2016-11
dc.description.abstractCellular models are important tools in various research areas related to colorectal biology and associated diseases. Herein, we review the most widely used cell lines and the different techniques to grow them, either as cell monolayer, polarized two-dimensional epithelia on membrane filters, or as three-dimensional spheres in scaffoldfree or matrix-supported culture conditions. Moreover, recent developments, such as gut-on-chip devices or the ex vivo growth of biopsy-derived organoids, are also discussed. We provide an overview on the potential applications but also on the limitations for each of these techniques, while evaluating their contribution to provide more reliable cellular models for research, diagnostic testing, or pharmacological validation related to colon physiology and pathophysiology.pt_PT
dc.description.sponsorshipWork in the authors’ laboratories is supported by Fundação para a Ciência e Tecnologia (FCT) through center Grant UID/MULTI/04046/2013 (to BioISI), by research grants from FCT, Portugal (PTDC/BIM-MEC/2131/2014), CFF-Cystic Fibrosis Foundation, USA (AMARAL15XX0, AMARAL15XX1), Gilead GÉNESE-Portugal Programme (PGG/008/2015); CF Trust, UK (SRC 003) to MDA, and from Portuguese association for inflammatory bowel disease (GEDII 2013), Portuguese association Maratona da Saúde (Cancro 2014), Portugal to PJ. J.F.P. was supported by fellowships BRJ-DGH 2012_oncologia from Instituto Nacional de Saúde Doutor Ricardo Jorge (Lisbon, Portugal) and BD/109162/2015 from FCT.pt_PT
dc.identifier.citationCell Mol Life Sci. 2016 Nov;73(21):3971-89. doi: 10.1007/s00018-016-2258-2. Epub 2016 May 4pt_PT
dc.identifier.doi10.1007/s00018-016-2258-2pt_PT
dc.identifier.issn1420-682X
dc.identifier.urihttp://hdl.handle.net/10400.18/4012
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlag/Birkhäuser Basept_PT
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00018-016-2258-2pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVias de Transdução de Sinal e Patologias Associadaspt_PT
dc.subjectColorectal Cancerpt_PT
dc.subjectInflammatory Bwel Diseasept_PT
dc.subjectCystic Fibrosispt_PT
dc.subjectOrganoidspt_PT
dc.subject3D culturept_PT
dc.subjectModel Systemspt_PT
dc.titleThe third dimension: new developments in cell culture models for colorectal researchpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.endPage3989pt_PT
oaire.citation.issue21pt_PT
oaire.citation.startPage3971pt_PT
oaire.citation.titleCellular and Molecular Life Sciencespt_PT
oaire.citation.volume73(21)pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationdc84f768-e6f2-4eea-b294-6c8ebbd1a156
relation.isProjectOfPublication.latestForDiscoverydc84f768-e6f2-4eea-b294-6c8ebbd1a156

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Review_3D colorectal cell models_ CMLS 2016.pdf
Size:
3.04 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DGH - Artigos em revistas internacionais