Publication
Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
| dc.contributor.author | Gonzalez-Diaz, Aida | |
| dc.contributor.author | Carrera-Salinas, Anna | |
| dc.contributor.author | Pinto, Miguel | |
| dc.contributor.author | Cubero, Meritxell | |
| dc.contributor.author | van der Ende, Arie | |
| dc.contributor.author | Langereis, Jeroen D. | |
| dc.contributor.author | Domínguez, M. Ángeles | |
| dc.contributor.author | Ardanuy, Carmen | |
| dc.contributor.author | Bajanca-Lavado, Paula | |
| dc.contributor.author | Marti, Sara | |
| dc.date.accessioned | 2022-12-02T15:36:18Z | |
| dc.date.available | 2022-12-02T15:36:18Z | |
| dc.date.issued | 2022-02-24 | |
| dc.description | Comparative Study | pt_PT |
| dc.description.abstract | Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation. | pt_PT |
| dc.description.sponsorship | Tis study was funded by Instituto de Salud Carlos III (ISCIII) through the Projects from the Fondo de Investigaciones Sanitarias “PI16/00977” to SM, and CIBER de Enfermedades Respiratorias (CIBERES–CB06/06/0037), co-funded by the European Regional Development Fund/European Social Fund (ERDF/ESF, “Investing in your future”), and CERCA Programme/Generalitat de Catalunya for institutional support. Bioinformatic analysis was supported by an Amazon Web Services (AWS) research grant to SM. AC was supported by FPU grant “FPU16/02202” (Formación de Profesorado Universitario, Ministerio de Educación, Spain), and SM was supported by Miguel Servet contract “CP19/00096” (ISCIII). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Sci Rep. 2022 Feb 24;12(1):3189. doi: 10.1038/s41598-022-07185-5 | pt_PT |
| dc.identifier.doi | 10.1038/s41598-022-07185-5 | pt_PT |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/8373 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Research | pt_PT |
| dc.relation.publisherversion | https://www.nature.com/articles/s41598-022-07185-5 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Haemophilus influenzae | pt_PT |
| dc.subject | Genetic Diversity | pt_PT |
| dc.subject | Serotype f | pt_PT |
| dc.subject | Multi-locus Sequence Type | pt_PT |
| dc.subject | Serotyping | pt_PT |
| dc.subject | Whole Genome Sequencing | pt_PT |
| dc.subject | Infecções Respiratórias | pt_PT |
| dc.title | Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 3189 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 12 | pt_PT |
| rcaap.embargofct | Acesso de acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |