A carregar...
Publicação
Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.4 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation.
Descrição
Comparative Study
Palavras-chave
Haemophilus influenzae Genetic Diversity Serotype f Multi-locus Sequence Type Serotyping Whole Genome Sequencing Infecções Respiratórias
Contexto Educativo
Citação
Sci Rep. 2022 Feb 24;12(1):3189. doi: 10.1038/s41598-022-07185-5
Editora
Nature Research