Publication
Ultra-high pressure LC for astaxanthin determination in shrimp by-products and active food packaging
| dc.contributor.author | Sanches-Silva, A. | |
| dc.contributor.author | Ribeiro, T. | |
| dc.contributor.author | Albuquerque, T.G. | |
| dc.contributor.author | Paseiro, P. | |
| dc.contributor.author | Sendón, R. | |
| dc.contributor.author | Bernaldo de Quirós, A. | |
| dc.contributor.author | López-Cervantes, J. | |
| dc.contributor.author | Sánchez-Machado, D. | |
| dc.contributor.author | Soto Valdez, H. | |
| dc.contributor.author | Angulo, I. | |
| dc.contributor.author | Pardo Aurrekoetxea, G. | |
| dc.contributor.author | Costa, H.S. | |
| dc.date.accessioned | 2013-02-12T13:10:12Z | |
| dc.date.available | 2013-02-12T13:10:12Z | |
| dc.date.issued | 2012-12-07 | |
| dc.description.abstract | Nowadays, there is increasing interest in natural antioxidants from food by-products. Astaxanthin is a potent antioxidant and one of the major carotenoids in crustaceans and salmonids. An ultra-high pressure liquid chromatographic method was developed and validated for the determination of astaxanthin in shrimp by-products, and its migration from new packaging materials to food simulants was also studied. The method uses an UPLCW BEH guard-column (2.1 x 5 mm, 1.7mm particle size) and an UPLCW BEH analytical column (2.1 x 50 mm, 1.7mm particle size). Chromatographic separation was achieved using a programmed gradient mobile phase consisting of (A) acetonitrile–methanol (containing 0.05 M ammonium acetate)–dichloromethane (75:20:5, v/v/v) and (B) ultrapure water. This method was evaluated with respect to validation parameters such as linearity, precision, limit of detection, limit of quantification and recovery. Low-density polyethylene films were prepared with different amounts of the lipid fraction of fermented shrimp waste by extrusion, and migration was evaluated into food simulants (isooctane and ethanol 95%, v/v). Migration was not detected under the tested conditions. | por |
| dc.description.sponsorship | This work was funded under Project no. 95935 by FONCICYT C002-2008-1/ALA – 127 249. The authors are grateful for the postdoctoral contract of Ana Sanches Silva in the frame of the Program ‘Science 2007’, funded by Fundação para a Ciência e a Tecnologia, Portugal. | por |
| dc.identifier.citation | Biomed Chromatogr. 2012 Dec 7. doi: 10.1002/bmc.2856. [Epub ahead of print] | por |
| dc.identifier.issn | 0269-3879 | |
| dc.identifier.other | ESSN: 1099-0801 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1288 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | John Wiley and Sons | por |
| dc.relation | Project no. 95935 by FONCICYT C002-2008-1/ALA – 127 249 | por |
| dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/bmc.2856/abstract;jsessionid=A0024DC54C4EEE9147B7B8AC75933E83.d04t04 | por |
| dc.subject | Astaxanthin | por |
| dc.subject | Shrimp by-products | por |
| dc.subject | Migration | por |
| dc.subject | Ultra-high Pressure Liquid Chromatography | por |
| dc.subject | Segurança Alimentar | por |
| dc.title | Ultra-high pressure LC for astaxanthin determination in shrimp by-products and active food packaging | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Biomedical Chromatography | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |