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In vitro bioacessibility and transport across Caco-2 monolayers of haloacetic acids in drinking water

2016-10Journal article Restricted access
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dc.contributor.authorMelo, A.
dc.contributor.authorFaria, M.A.
dc.contributor.authorPinto, E.
dc.contributor.authorMansilha, C.
dc.contributor.authorFerreira, I.M.P.L.V.O.
dc.date.accessioned2017-03-07T15:56:32Z
dc.date.available2021-11-01T01:30:09Z
dc.date.issued2016-10
dc.description.abstractWater disinfection plays a crucial role in water safety but it is also a matter of concern as the use of disinfectants promotes the formation of disinfection by-products (DBPs). Haloacetic acids (HAAs) are one of the major classes of DBPs since they are frequently found in treated water, are ubiquitous, pervasive and have high water solubility, so a great concern emerged about their formation, occurrence and toxicity. Exposure to HAAs is influenced by consumption patterns and diet of individuals thus their bioavailability is an important parameter to the overall toxicity. In the current study the bioacessibility of the most representative HAAs (chloroacetic acid - MCAA, bromoacetic acid - MBAA, dichloroacetic acid - DCAA, dibromoacetic acid - DBAA, and trichloroacetic acid - TCAA) after simulated in vitro digestion (SIVD) in tap water and transport across Caco-2 monolayers was evaluated. Compounds were monitored in 8 points throughout the digestion phases by an optimized LC-MS/MS methodology. MCAA and MBAA were not bioaccessible after SIVD whereas DCAA, DBAA and TCAA are highly bioaccessible (85 ± 4%, 97 ± 4% and 106 ± 7% respectively). Concerning transport assays, DCAA and DBAA were highly permeable throughout the Caco-2 monolayer (apparent permeability and calculated fraction absorbed of 13.62 × 10(-6) cm/s and 90% for DCAA; and 8.82 × 10(-6) cm/s and 84% for DBAA), whereas TCAA showed no relevant permeability. The present results may contribute to efficient risk analysis studies concerning HAAs oral exposure from tap water taking into account the different biological behaviour of these chemically similar substances.pt_PT
dc.description.sponsorshipThis work received financial support from the project UID/QUI/50006/2013- POCI/01/0145/FEDER/007265 with support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020. A. Melo and M. A. Faria wishes to thank the Fundaçao para a Ciência Tecnologia for grants SFRH/BPD/86898/2012 and SFRH/BPD/110356/2015, respectively. E. Pinto thanks the project NORTE-01-0145-FEDER-000011 e Qualidade e Segurança Alimentar d uma abordagem (nano) tecnologica.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationChemosphere. 2016 Oct;161:19-26. doi: 10.1016/j.chemosphere.2016.06.088. Epub 2016 Jul 10pt_PT
dc.identifier.doi10.1016/j.chemosphere.2016.06.088pt_PT
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.18/4548
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/Pergamonpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0045653516308463pt_PT
dc.subjectAcetatespt_PT
dc.subjectCaco-2 Cellspt_PT
dc.subjectCell Membrane Permeabilitypt_PT
dc.subjectDichloroacetic Acidpt_PT
dc.subjectDisinfectionpt_PT
dc.subjectDrinking Waterpt_PT
dc.subjectHumanspt_PT
dc.subjectTandem Mass Spectrometrypt_PT
dc.subjectTrichloroacetic Acidpt_PT
dc.subjectWater Pollutants, Chemicalpt_PT
dc.subjectWater Purificationpt_PT
dc.subjectWater Supplypt_PT
dc.subjectÁgua e Solopt_PT
dc.titleIn vitro bioacessibility and transport across Caco-2 monolayers of haloacetic acids in drinking waterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage26pt_PT
oaire.citation.startPage19pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume161pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd7883dc7-6335-4f26-8bd0-e2ea4bb07c07
relation.isProjectOfPublication.latestForDiscoveryd7883dc7-6335-4f26-8bd0-e2ea4bb07c07

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