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Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping—a tool for hospital infection surveillance

2015-10Journal article Open access
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dc.contributor.authorSabino, Raquel
dc.contributor.authorSampaio, P.
dc.contributor.authorRosado, L.
dc.contributor.authorVideira, Z.
dc.contributor.authorGrenouillet, F.
dc.contributor.authorPais, C.
dc.date.accessioned2016-02-17T18:01:01Z
dc.date.available2016-02-17T18:01:01Z
dc.date.issued2015-10
dc.description.abstractCandida parapsilosis emerged as an important opportunistic pathogen, causing candidaemia worldwide. Nosocomial outbreaks triggered by this species have been frequently described, particularly in cancer patients. For a better understanding of its epidemiology, several typing methods are used and microsatellite analysis has been reported as highly discriminant. The main objective of this work was to study C. parapsilosis isolates by application of microsatellite genotyping to distinguish epidemiologically related strains, compare clinical and environmental isolates and determine possible routes of dispersion of the isolates in the hospital setting. A total of 129 C. parapsilosis isolates from different origins, including hospital environment and hands of healthcare workers, were genotyped using four microsatellite markers. The isolates were recovered from different health institutions. Analysis of C. parapsilosis isolates from hospital environment showed great genotypic diversity; however, the same or very similar genotypes were also found. The same multilocus genotype was shared by isolates recovered from the hand of a healthcare worker, from the hospital environment and from patients of the same healthcare institution, suggesting that these could be possible routes of transmission and that infections due to C. parapsilosis may be mainly related with exogenous transmission to the patient. Examination of sequential isolates from the same patients showed that colonizing and bloodstream isolates had the same multilocus genotype in the majority of cases. We demonstrate that this typing method is able to distinguish clonal clusters from genetically unrelated genotypes and can be a valuable tool to support epidemiologic investigations in the hospital setting.pt_PT
dc.identifier.citationClin Microbiol Infect. 2015 Oct;21(10):954.e1-8. doi: 10.1016/j.cmi.2015.06.001. Epub 2015 Jun 10pt_PT
dc.identifier.doi10.1016/j.cmi.2015.06.001pt_PT
dc.identifier.issn1198-743X
dc.identifier.urihttp://hdl.handle.net/10400.18/3395
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley/ European Society of Clinical Microbiology and Infectious Diseasespt_PT
dc.subjectCandida Parapsilosispt_PT
dc.subjectHealthcare Workerspt_PT
dc.subjectHospital Air and Surfacespt_PT
dc.subjectMicrosatelliteGenotypingpt_PT
dc.subjectNosocomial Infectionspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleAnalysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping—a tool for hospital infection surveillancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage954.e8pt_PT
oaire.citation.startPage954.e1pt_PT
oaire.citation.titleClinical Microbiology and Infectionpt_PT
oaire.citation.volume21(10)pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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