Publication
Snapshot of resistome, virulome and mobilome in aquaculture
| dc.contributor.author | Salgueiro, Vanessa | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Rosado, Tânia | |
| dc.contributor.author | Bandarra, Narcisa M. | |
| dc.contributor.author | Botelho, Maria João | |
| dc.contributor.author | Dias, Elsa | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2024-01-25T12:00:09Z | |
| dc.date.available | 2024-01-25T12:00:09Z | |
| dc.date.issued | 2023-12 | |
| dc.description.abstract | Aquaculture environments can be hotspots for resistance genes through the surrounding environment. Our objective was to study the resistome, virulome and mobilome of Gram-negative bacteria isolated in seabream and bivalve molluscs, using a WGS approach. Sixty-six Gram-negative strains (Aeromonadaceae, Enterobacteriaceae, Hafniaceae, Morganellaceae, Pseudomonadaceae, Shewanellaceae, Vibrionaceae, and Yersiniaceae families) were selected for genomic characterization. The species and MLST were determined, and antibiotic/disinfectants/heavy metals resistance genes, virulence determinants, MGE, and pathogenicity to humans were investigated. Our study revealed new sequence-types (e.g. Aeromonas spp. ST879, ST880, ST881, ST882, ST883, ST887, ST888; Shewanella spp. ST40, ST57, ST58, ST60, ST61, ST62; Vibrio spp. ST206, ST205). >140 different genes were identified in the resistome of seabream and bivalve molluscs, encompassing genes associated with β-lactams, tetracyclines, aminoglycosides, quinolones, sulfonamides, trimethoprim, phenicols, macrolides and fosfomycin resistance. Disinfectant resistance genes qacE-type, sitABCD-type and formA-type were found. Heavy metals resistance genes mdt, acr and sil stood out as the most frequent. Most resistance genes were associated with antibiotics/disinfectants/heavy metals commonly used in aquaculture settings. We also identified 25 different genes related with increased virulence, namely associated with adherence, colonization, toxins production, red blood cell lysis, iron metabolism, escape from the immune system of the host. Furthermore, 74.2 % of the strains analysed were considered pathogenic to humans. We investigated the genetic environment of several antibiotic resistance genes, including blaTEM-1B, blaFOX-18, aph(3″)-Ib, dfrA-type, aadA1, catA1-type, tet(A)/(E), qnrB19 and sul1/2. Our analysis also focused on identifying MGE in proximity to these genes (e.g. IntI1, plasmids and TnAs), which could potentially facilitate the spread of resistance among bacteria across different environments. This study provides a comprehensive examination of the diversity of resistance genes that can be transferred to both humans and the environment, with the recognition that aquaculture and the broader environment play crucial roles as intermediaries within this complex transmission network. | pt_PT |
| dc.description.abstract | Highlights: - New STs (17) and possible evolutionary relationships with other STs were identified. - Over 140 resistance genes provided a snapshot of the aquaculture resistome. - Many resistance genes found are common to those of clinical isolates (e.g. qnrB19). - Many ARGs detected (e.g. sul) are associated to antibiotics used in aquaculture. - Several (74.2 %) strains studied were classified as pathogenic to human. | pt_PT |
| dc.description.sponsorship | V.S. has a Ph.D. fellowship granted by the FCT (Fundação para a Ciência e a Tecnologia) with the reference SFRH/BD/133100/2017 cofinanced by European Social Fund and the Operational Program for Human Capital (POCH), Portugal. This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 773830: One Health European Joint Programme (WORLDCOM project), and by FCT/MCTES [UIDB/ 00211/2020] through national funds. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Sci Total Environ. 2023 Dec 20:905:166351. doi: 10.1016/j.scitotenv.2023.166351. Epub 2023 Aug 19 | pt_PT |
| dc.identifier.doi | 10.1016/j.scitotenv.2023.166351 | pt_PT |
| dc.identifier.issn | 0048-9697 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/8981 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | Decrypting the diversity of microbiome in aquaculture | |
| dc.relation | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| dc.relation | Center for the Study of Animal Science | |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0048969723049768?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Bivalve Molluscs | pt_PT |
| dc.subject | Gram-negative bacteria | pt_PT |
| dc.subject | Mobilome | pt_PT |
| dc.subject | Resistome | pt_PT |
| dc.subject | Seabream | pt_PT |
| dc.subject | Virulome | pt_PT |
| dc.subject | Aquaculture | pt_PT |
| dc.subject | Disinfectants | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.subject | Agentes Microbianos e Ambiente | pt_PT |
| dc.title | Snapshot of resistome, virulome and mobilome in aquaculture | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Decrypting the diversity of microbiome in aquaculture | |
| oaire.awardTitle | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| oaire.awardTitle | Center for the Study of Animal Science | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133100%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/773830/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00211%2F2020/PT | |
| oaire.citation.startPage | 166351 | pt_PT |
| oaire.citation.title | Science of The Total Environment | pt_PT |
| oaire.citation.volume | 905 | pt_PT |
| oaire.fundingStream | OE | |
| oaire.fundingStream | H2020 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | European Commission | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.embargofct | Acesso de acordo com a política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | b16cfc8b-bb9b-43b9-9b11-2c49a0856050 | |
| relation.isProjectOfPublication | 94d118fb-33ce-49fa-b1ed-d5bddf63581d | |
| relation.isProjectOfPublication | 69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7 | |
| relation.isProjectOfPublication.latestForDiscovery | 94d118fb-33ce-49fa-b1ed-d5bddf63581d |