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Exploring flagellar contributions to motility and virulence in Arcobacter butzleri

2024-10-26Clinical study Restricted access
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datacite.subject.fosCiências Naturais
dc.contributor.authorSantos, Raquel
dc.contributor.authorMateus, Cristiana
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2025-04-02T12:45:58Z
dc.date.available2025-04-02T12:45:58Z
dc.date.issued2024-10-26
dc.description.abstractFlagella is a well-known bacterial structure crucial for motility, which also plays pivotal roles in pathogenesis. Arcobacter butzleri, an enteropathogen, possesses a distinctive polar flagellum whose functional aspects remain largely unexplored. Upon investigating the factors influencing A. butzleri motility, we uncovered that environmental conditions like temperature, oxygen levels, and nutrient availability play a significant role. Furthermore, compounds that are found in human gut, such as short-chain fatty acids, mucins and bile salts, have a role in modulating the motility, and in turn, the pathogenicity of A. butzleri. Further investigation demonstrated that A. butzleri ΔflaA mutant showed a reduction in motility with a close to null average velocity, as well as a reduction on biofilm formation. In addition, compared with the wild-type, the ΔflaA mutant showed a decreased ability to invade Caco-2 cells and to adhere to mucins. Taken together, our findings support the role of environmental conditions and gut host associated compounds influencing key physiological aspects of the gastrointestinal pathogen A. butzleri, such as motility, and support the role of the flagellum on bacterial virulence.eng
dc.description.sponsorshipCristiana Mateus is recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). Susana Ferreira acknowledges UBI and FCT by the contract of Scientific Employment according to DL57/2016. The microscopy facility used in the development of this work is part of the PPBI-Portuguese Platform of BioImaging and is partially supported by the Project POCI-01-0145-FEDER-022122.. This work was supported by funds from the CICS-UBI base funding with DOI https://doi.org/10.54499/UIDB/00709/2020 (https://doi.org/10.54499/UIDB/00709/2020) and program funding with DOI https://doi.org/10.54499/UIDP/00709/2020 (https://doi.org/10.54499/UIDP/00709/2020) with national funds registered in the budget of the Foundation for Science and Technology.
dc.identifier.citationWorld J Microbiol Biotechnol. 2024 Oct 26;40(11):367. doi: 10.1007/s11274-024-04175-9
dc.identifier.doi10.1007/s11274-024-04175-9
dc.identifier.issn0959-3993
dc.identifier.pmid39455472
dc.identifier.urihttp://hdl.handle.net/10400.18/10465
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationUI/BD/151023/2021
dc.relationUIDB/00709/2020
dc.relationUIDB/00709/2020
dc.relationUIDP/00709/2020
dc.relation.hasversionhttps://link.springer.com/article/10.1007/s11274-024-04175-9
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectArcobacter butzleri
dc.subjectEnvironmental Conditions
dc.subjectFlagellum
dc.subjectHost
dc.subjectMotility
dc.subjectVirulence
dc.subjectInfecções Gastrointestinais
dc.titleExploring flagellar contributions to motility and virulence in Arcobacter butzlerieng
dc.typeclinical study
dspace.entity.typePublication
oaire.citation.issue11
oaire.citation.startPage367
oaire.citation.titleWorld Journal of Microbiology and Biotechnology
oaire.citation.volume40
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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