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Characterizing Antimicrobial Resistance in Clinically Relevant Bacteria Isolated at the Human/Animal/Environment Interface Using Whole-Genome Sequencing in Austria

2022-09-24Journal article Open access
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dc.contributor.authorCabal, Adriana
dc.contributor.authorRab, Gerhard
dc.contributor.authorDaza-Prieto, Beatriz
dc.contributor.authorStöger, Anna
dc.contributor.authorPeischl, Nadine
dc.contributor.authorChakeri, Ali
dc.contributor.authorMo, Solveig Sølverød
dc.contributor.authorBock, Harald
dc.contributor.authorFuchs, Klemens
dc.contributor.authorSucher, Jasmin
dc.contributor.authorRathammer, Krista
dc.contributor.authorHasenberger, Petra
dc.contributor.authorStadtbauer, Silke
dc.contributor.authorCaniça, Manuela
dc.contributor.authorStrauß, Peter
dc.contributor.authorAllerberger, Franz
dc.contributor.authorWögerbauer, Markus
dc.contributor.authorRuppitsch, Werner
dc.date.accessioned2023-03-20T12:02:38Z
dc.date.available2023-03-20T12:02:38Z
dc.date.issued2022-09-24
dc.descriptionThis article belongs to the Collection Feature Papers in Molecular Microbiology.pt_PT
dc.description.abstractAntimicrobial resistance (AMR) is a public health issue attributed to the misuse of antibiotics in human and veterinary medicine. Since AMR surveillance requires a One Health approach, we sampled nine interconnected compartments at a hydrological open-air lab (HOAL) in Austria to obtain six bacterial species included in the WHO priority list of antibiotic-resistant bacteria (ARB). Whole genome sequencing-based typing included core genome multilocus sequence typing (cgMLST). Genetic and phenotypic characterization of AMR was performed for all isolates. Eighty-nine clinically-relevant bacteria were obtained from eight compartments including 49 E. coli, 27 E. faecalis, 7 K. pneumoniae and 6 E. faecium. Clusters of isolates from the same species obtained in different sample collection dates were detected. Of the isolates, 29.2% were resistant to at least one antimicrobial. E. coli and E. faecalis isolates from different compartments had acquired antimicrobial resistance genes (ARGs) associated with veterinary drugs such as aminoglycosides and tetracyclines, some of which were carried in conjugative and mobilizable plasmids. Three multidrug resistant (MDR) E. coli isolates were found in samples from field drainage and wastewater. Early detection of ARGs and ARB in natural and farm-related environments can identify hotspots of AMR and help prevent its emergence and dissemination along the food/feed chain.pt_PT
dc.description.sponsorshipThis research was partially funded by the European Union’s Horizon 2020 Research and Innovation program, grant agreement No 773830: One Health European Joint Program.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2022 Sep 24;23(19):11276. doi: 10.3390/ijms231911276pt_PT
dc.identifier.doi10.3390/ijms231911276pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/8568
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/19/11276pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectAntimicrobial Resistance Genespt_PT
dc.subjectOne Healthpt_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleCharacterizing Antimicrobial Resistance in Clinically Relevant Bacteria Isolated at the Human/Animal/Environment Interface Using Whole-Genome Sequencing in Austriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/773830/EU
oaire.citation.issue19pt_PT
oaire.citation.startPage11276pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication94d118fb-33ce-49fa-b1ed-d5bddf63581d
relation.isProjectOfPublication.latestForDiscovery94d118fb-33ce-49fa-b1ed-d5bddf63581d

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