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Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs

2021-09-07Journal article Restricted access
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dc.contributor.authorFurtado, R.
dc.contributor.authorCoelho, A.
dc.contributor.authorMorais, M.
dc.contributor.authorLeitão, A.L.
dc.contributor.authorSaraiva, M.
dc.contributor.authorCorreia, C.B.
dc.contributor.authorBatista, R.
dc.date.accessioned2021-12-20T16:12:50Z
dc.date.available2021-12-20T16:12:50Z
dc.date.issued2021-09-07
dc.descriptionWe thank AMBIFOOD, Lda, for providing the reagent kits and all the necessary equipment for the PCR methodolog.pt_PT
dc.descriptionThe online version contains supplementary material available at https://doi.org/10.1007/s12161-021-02114-0.pt_PT
dc.description.abstractIn the European Union (EU), Salmonella was the causative agent responsible for almost one in three (30.7%) of all foodborne outbreaks reported by member states during 2018, causing 11,581 cases of illness, which represented an increase of 20.6% compared to 2017. Considering the importance of this foodborne zoonotic bacterium in food safety and human health, several strategies for the control and consequent detection of Salmonella in foodstuffs are continuously being developed. In this study, we have tested 137 food samples (78 potentially naturally contaminated, 21 artificially contaminated with high levels of Salmonella, and 38 artificially contaminated with low levels of Salmonella) in order to compare the results and performance of three Salmonella detection methods: standard conventional culture (ISO 6579–1), SureFast® Salmonella ONE real-time PCR, and VIDAS® (Vitek Immunodiagnostic Assay System) Easy SLM, an Enzyme Linked Fluorescent Assay (ELFA). Although SureFast® Salmonella ONE real-time PCR was the fastest, it showed more inconclusive results, due to PCR inhibition and false positive results. ISO and VIDAS® protocols gave identical results and proved to be more robust than SureFast® Salmonella ONE real-time PCR when testing different food matrices, despite its longer response times. SureFast® Salmonella ONE real-time PCR may be appropriate to be used when the objective is to test food matrices that are known not to interfere with PCR and expected to be negative for Salmonella. All the analytical tested methods have advantages and limitations and thus, depending on the situation, may be used as the elected method for Salmonella detection in foodstuffs in accordance with the purpose of the laboratorial analysis.pt_PT
dc.description.sponsorshipThis work was funded by Instituto Nacional de Saúde Dr. Ricardo Jorgept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Anal Methods. 2021. Epub 07 September 2021. doi: 10.1007/s12161-021-02114-0pt_PT
dc.identifier.doi10.1007/s12161-021-02114-0pt_PT
dc.identifier.eissn1936-976X
dc.identifier.issn1936-9751
dc.identifier.urihttp://hdl.handle.net/10400.18/7824
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s12161-021-02114-0pt_PT
dc.subjectFoodstufspt_PT
dc.subjectSalmonella Detection ·pt_PT
dc.subjectConventional and Alternative Methodspt_PT
dc.subjectFood Safetypt_PT
dc.subjectSalmonella spppt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleComparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood Analytical Methodspt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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