Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs

Furtado, R.; Coelho, A.; Morais, M.; Leitão, A.L.; Saraiva, M.; Correia, C.B.; Batista, R.

http://hdl.handle.net/10400.18/7824

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Comparison of ISO 6579–1 VIDAS Easy SLM, and SureFast Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs.pdf		900.94 KB	Adobe PDF	Download

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Abstract(s)

In the European Union (EU), Salmonella was the causative agent responsible for almost one in three (30.7%) of all foodborne outbreaks reported by member states during 2018, causing 11,581 cases of illness, which represented an increase of 20.6% compared to 2017. Considering the importance of this foodborne zoonotic bacterium in food safety and human health, several strategies for the control and consequent detection of Salmonella in foodstuffs are continuously being developed. In this study, we have tested 137 food samples (78 potentially naturally contaminated, 21 artificially contaminated with high levels of Salmonella, and 38 artificially contaminated with low levels of Salmonella) in order to compare the results and performance of three Salmonella detection methods: standard conventional culture (ISO 6579–1), SureFast® Salmonella ONE real-time PCR, and VIDAS® (Vitek Immunodiagnostic Assay System) Easy SLM, an Enzyme Linked Fluorescent Assay (ELFA). Although SureFast® Salmonella ONE real-time PCR was the fastest, it showed more inconclusive results, due to PCR inhibition and false positive results. ISO and VIDAS® protocols gave identical results and proved to be more robust than SureFast® Salmonella ONE real-time PCR when testing different food matrices, despite its longer response times. SureFast® Salmonella ONE real-time PCR may be appropriate to be used when the objective is to test food matrices that are known not to interfere with PCR and expected to be negative for Salmonella. All the analytical tested methods have advantages and limitations and thus, depending on the situation, may be used as the elected method for Salmonella detection in foodstuffs in accordance with the purpose of the laboratorial analysis.

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We thank AMBIFOOD, Lda, for providing the reagent kits and all the necessary equipment for the PCR methodolog.
The online version contains supplementary material available at https://doi.org/10.1007/s12161-021-02114-0.