A carregar...
Publicação
Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles.
Orientador(es)
Resumo(s)
Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs.
Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs on living cells. In the present
work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet assay) of three cSLN
formulations (A–C) were evaluated in HepG2 and Caco-2 cells. cSLN showed an average hydrodynamic diameter (z-ave) of
141–222 nm, zeta-potential of 55.0–72.5mV and polidispersity indices (PdI) of 0.336–0.421. Dispersion in physiological
buffers increased z-ave and PdI. 0.01mgml–1 cSLN unaffected cell viability, but 1.0mgml–1 significantly decreased it, being
cSLN-C (Compritol-based) the most toxic and HepG2 the most affected. DNA damage was not significantly increased by
0.1mgml–1 cSLN but damage was observed at 1.0mgml–1 cSLN-C. Thus, no genotoxicity is to be expected at concentrations
that do not reduce cell viability.
Descrição
Palavras-chave
Comet Assay Caco-2 HepG2 Genotoxicity Solid Lipid Nanoparticles Ar e Saúde Ocupacional Genotoxidade Ambiental e Ocupacional
Contexto Educativo
Citação
J Appl Toxicol. 2014 Apr;34(4):395-403. doi: 10.1002/jat.2961. Epub 2013 Nov 15.
Editora
John Wiley & Sons, Ltd