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Titanium dioxide nanomaterials - induced DNA damage in intestinal cells following simulated in vitro digestion

2021-04-20Conference object Restricted access
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dc.contributor.authorVieira, Adriana
dc.contributor.authorRolo, Dora
dc.contributor.authorVital, Nádia
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorAlvito, Paula
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-01-14T15:05:50Z
dc.date.available2022-01-14T15:05:50Z
dc.date.issued2021-04-20
dc.description.abstractIntroduction: The increased use of titanium dioxide nanomaterials (TiO2) in food products has raised oral exposure to those nanomaterials, with subsequent risks to human health, particularly genotoxicity and, ultimately, cancer development. In humans, the digestion process may modify the physicochemical properties of TiO2, thereby shaping the potential biological outcomes. Thus, such process should be considered when assessing their hazard upon oral exposure. This work aimed to investigate the genotoxic effects of three TiO2 (NM-102, NM-103 and NM-105, JRC repository) after the simulation of the human digestive process using the standardized INFOGEST in vitro digestion method. The secondary physicochemical properties and DNA damage levels, using the comet assay, were analysed in two intestinal cell lines exposed for 24h to digested or undigested TiO2. Results: An increase in the level of DNA strand breaks in two intestinal cell lines(Caco-2 and HT29-MTX-E12) was observed after exposure to digested NM-105, concomitantly with a decrease in its hydrodynamic size, comparatively to the undigested nanomaterial. Moreover, the digested NM-103 induced DNA damage in Caco-2 cells whereas the undigested nanomaterial did not. The FPG-modified comet assay also revealed an increase in oxidative DNA lesions upon treatment of Caco-2 with NM-103 and HT29-MTX-E12 with NM-102. Conclusions: One of the digested TiO2(NM-105) can be classified as potentially genotoxic in both cell lines, while the digested NM-103 induced an equivocal genotoxic response in Caco-2 cells. Therefore, the digestion simulation is of relevance to investigate the potential genotoxic effects of ingested nanomaterials.pt_PT
dc.description.sponsorshipFCT/MCTES (PTDC/SAU-PUB/29481/2017); ToxOmics(UIDB/00009/2020); iMed.ULisboa(UIDB/04138/2020, CEECIND/03143/2017); CESAM (UIDP/50017/2020+UIDB/50017/2020).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7853
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCEECIND/03143/2017pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationResearch Institute for Medicines
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleTitanium dioxide nanomaterials - induced DNA damage in intestinal cells following simulated in vitro digestionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title10th International Conference on Nanotoxicology (NANOTOX), 20-22 april 2021pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRolo
person.familyNameVital
person.familyNameCampos Simão Bettencourt
person.familyNameSilva
person.familyNameLouro
person.givenNameDora
person.givenNameNádia
person.givenNameAna Francisca
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7E16-CFCA-B797
person.identifier.ciencia-id7C1F-F932-33DC
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0003-4327-2279
person.identifier.orcid0000-0002-3391-0268
person.identifier.orcid0000-0002-8498-5892
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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