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Impact of DINCH® in human cells: evaluation of its potential cytotoxic and genotoxic effects

2018-05-11Conference object Restricted access
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dc.contributor.authorVasconcelos, Ana Luisa
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2018-08-16T14:30:26Z
dc.date.available2018-08-16T14:30:26Z
dc.date.issued2018-05-11
dc.description.abstractThe chemical Di-(iso-nonyl)-cyclohexane-1,2-dicarboxylate (DINCH) has been applied as a non-aromatic plasticizer and substitute for other phthalate plasticizers such as di-(2-ethylhexyl) phthalate (DEHP) and di-(iso-nonyl) phthalate (DINP), that have shown to have adverse effects. Since DINCH detected in indoor dust has increased after the market introduction of this plasticizer in 2002, the human exposure is a concern. Health-related guidance values have been derived for children and adults, namely 3 mg/L and 4.5 mg/L of DINCH metabolites in the urine, respectively. Recently, the exposure of Portuguese children to DINCH was reported, in spite the low levels detected in children’s urine, which were below the established health guidance levels. Conversely, few studies have addressed the potential toxicity of DINCH but in vivo studies suggest its bioavailability, leading to concerns in respect to systemic exposure or longer term consequences of its use, namely to liver or kidney cells. To contribute to the hazard characterization of DINCH, its potential cytotoxicity and genotoxicity was investigated in human liver cells, following the exposure of HepG2 cells to a range of concentrations of this chemical agent. The methodology included the MTT assay for cytoxicity determination, the comet assay for the detection of DNA damage and the micronucleus assay for determination of chromosomal damage, based on the OECD TG 487 guideline (2016). The results showed that concentrations ranging from 1 to 500 µg/mL were neither cytotoxic following 24h exposure of HepG2 cells, nor had impact on DNA or chromosome damage. Underway studies focus on the effects under the presence of exogenous liver metabolic enzymes (S9 fraction) and on the detection of oxidative DNA damage. Further ongoing investigation is addressing the potential nephrotoxic effects of DINCH using kidney cells.pt_PT
dc.description.sponsorshipThis research was partially funded by INSA, Centre for Toxicogenomics and Human Health, ToxOmics (UID/MMIM/00009/2013). The authors thank BASF for kindly providing Hexamoll® DINCH for analysis.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/5598
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Nacional de Saúde Doutor Ricardo Jorge, IPpt_PT
dc.subjectEmergent Plasticizerspt_PT
dc.subjectRisk Assessmentpt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleImpact of DINCH® in human cells: evaluation of its potential cytotoxic and genotoxic effectspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.title1st Human Biomonitoring Workshop in Portugal “Bridging Chemical Exposure to Human Health”, INSA, 11 maio 2018pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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