Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD

Viegas, Olga; Esteves, Cristina; Rocha, Janete; Melo, Armindo; Ferreira, Isabel M.P.L.V.O.

http://hdl.handle.net/10400.18/7668

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Authors

Ferreira, Isabel M.P.L.V.O.

Abstract(s)

The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R2 > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL-1) and trans-RSV (169-2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method.

Highlights: Novel approach for simultaneous determination of melatonin and trans-resveratrol; The microextraction procedure is optimized by multivariate optimization strategy; Demonstration of the applicability of the final method in real samples; Novel method has potential for rapid analysis of melatonin and trans-resveratrol.

Keywords

Chromatography, High Pressure Liquid Food Analysis Liquid Phase Microextraction Melatonin Osmolar Concentration Resveratrol Solvents Time Factors Wine Composição dos Alimentos