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Fungal and microbial volatile organic compounds exposure assessment in a waste sorting plant
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Orientador(es)
Resumo(s)
In the management of solid waste, pollutants over a wide range are released with different
routes of exposure for workers. The potential for synergism among the pollutants raises
concerns about potential adverse health effects, and there are still many uncertainties
involved in exposure assessment. In this study, conventional (culture-based) and molecular
real-time polymerase chain reaction (RTPCR) methodologies were used to assess fungal
air contamination in a waste-sorting plant which focused on the presence of three potential
pathogenic/toxigenic fungal species: Aspergillus flavus, A. fumigatus, and Stachybotrys
chartarum. In addition, microbial volatile organic compounds (MVOC) were measured by
photoionization detection. For all analysis, samplings were performed at five different workstations
inside the facilities and also outdoors as a reference. Penicillium sp. were the most
common species found at all plant locations. Pathogenic/toxigenic species (A. fumigatus and
S. chartarum) were detected at two different workstations by RTPCR but not by culture-based
techniques. MVOC concentration indoors ranged between 0 and 8.9 ppm (average 5.3 ±
3.16 ppm). Our results illustrated the advantage of combining both conventional and molecular
methodologies in fungal exposure assessment. Together with MVOC analyses in indoor
air, data obtained allow for a more precise evaluation of potential health risks associated
with bioaerosol exposure. Consequently, with this knowledge, strategies may be developed
for effective protection of the workers.
Descrição
Palavras-chave
Fungi Molecular Detection Microbial Volatile Compounds Solid Waste Sorting
Contexto Educativo
Citação
J Toxicol Environ Health A. 2012;75(22-23):1410-7