Publication
Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, Portugal
| dc.contributor.author | Vilares, A. | |
| dc.contributor.author | Gargaté, M.J. | |
| dc.contributor.author | Ferreira, I. | |
| dc.contributor.author | Martins, S. | |
| dc.contributor.author | Júlio, C. | |
| dc.contributor.author | Waap, H. | |
| dc.contributor.author | Angelo, H. | |
| dc.contributor.author | Gomes, João Paulo | |
| dc.date.accessioned | 2015-09-24T15:54:05Z | |
| dc.date.available | 2015-09-24T15:54:05Z | |
| dc.date.issued | 2014-10-15 | |
| dc.description.abstract | Cats and pigeons are important factors in the epidemiology of Toxoplasma gondii as felidsare the only definitive hosts that can excrete environmentally resistant oocysts, andpigeons share the same places of cats and humans constituting a good model and indicatorof the ground field contamination. We aimed to study the virulence and genotypes ofT. gondii isolated from pigeons and stray cats in Lisbon, Portugal. Fresh samples of brainfrom 41 pigeons and 164 cats revealing antibodies to T. gondii were inoculated in mice.Three isolates (one isolated from a cat and two isolated from pigeons) were virulent in themouse model. Sag2-based genotyping of T. gondii was achieved in 70.7% (29/41) of samplesisolated from pigeons (26 samples were type II, two were type III, and one strain was type I).From the cat brain samples, 50% (82/164) yielded Sag2 positive results, where 72 belongedto genotype II and 10 were no type III (it was not possible to discriminate between typeI and II). Further genotyping was obtained by multiplex PCR of 5 microsatellites (TUB2,TgM-A, W35, B17, B18), allowing the identification of two recombinant strains that hadbeen previously identified as type II by Sag2 amplification (one isolated from cat brain andthe other from pigeon brain). This is the first evidence of recombinant strains circulatingin Portugal and the first report of T. gondii genotyping from cats in this country. This studyalso highlights the importance of environmental contamination in the synanthropic cycleconstituting a potential source of human infection. | por |
| dc.description.sponsorship | This research was supported by the Foundation for Science and Technology (FCT) – Ministry of Science, Technology and Higher Education (project reference – PTDC/SAU-ESA/70388/2006). The authors thank to Rogério Tenreiro for the tips in the presentation ofthe article,to the head of the Division of Sanitary Control of the Municipality of Lisbon, Dra. Luisa Costa Gomes, for the invaluable collaboration in this study. Luis Vieira, Sónia Pedro, Ana Cardoso, Assunc¸ ão António and Paula Grilo (INSA) for technical assistance. | por |
| dc.identifier.citation | Vet Parasitol. 2014 Oct 15;205(3-4):506-11. doi: 10.1016/j.vetpar.2014.08.006. Epub 2014 Aug 26 | por |
| dc.identifier.doi | 10.1016/j.vetpar.2014.08.006 | |
| dc.identifier.issn | 0304-4017 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/3166 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier/ American Association of Veterinary Parasitologists, the European Veterinary Parasitology College, World Association for the Advancement of Veterinary Parasitology | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0304401714004439 | por |
| dc.subject | Toxoplasma Gondii | por |
| dc.subject | Cats | por |
| dc.subject | Pigeons | por |
| dc.subject | Genotype | por |
| dc.subject | Infecções Sexualmente Transmissíveis | por |
| dc.title | Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, Portugal | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 511 | por |
| oaire.citation.startPage | 506 | por |
| oaire.citation.title | Veterinary Parasitology | por |
| oaire.citation.volume | 205(3-4) | por |
| rcaap.rights | embargoedAccess | por |
| rcaap.type | article | por |