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Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis

2022-10-15Journal article Open access
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dc.contributor.authorCochicho, Daniela
dc.contributor.authorNunes, Alexandra
dc.contributor.authorGomes, João Paulo
dc.contributor.authorMartins, Luís
dc.contributor.authorCunha, Mário
dc.contributor.authorMedeiros-Fonseca, Beatriz
dc.contributor.authorOliveira, Paula
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorMedeiros, Rui
dc.contributor.authorMendonça, Joana
dc.contributor.authorVieira, Luis
dc.contributor.authorGil da Costa, Rui M.
dc.contributor.authorFelix, Ana
dc.date.accessioned2023-01-31T12:45:59Z
dc.date.available2023-01-31T12:45:59Z
dc.date.issued2022-10-15
dc.descriptionThis article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics.pt_PT
dc.description.abstractThe study of ()-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. This study aimed to determine the HPV16 variant and sublineage present in the K14HPV16 mouse model. This information can be considered of great importance to further enhance this K14HPV16 model as an essential research tool and optimize its use for basic and translational studies. Our study evaluated HPV DNA from 17 samples isolated from 4 animals, both wild-type (n = 2) and HPV16-transgenic mice (n = 2). Total DNA was extracted from tissues and the detection of HPV16 was performed using a qPCR multiplex. HPV16-positive samples were subsequently whole-genome sequenced by next-generation sequencing techniques. The phylogenetic positioning clearly shows K14HPV16 samples clustering together in the sub-lineage A1 (NC001526.4). A comparative genome analysis of K14HPV16 samples revealed three mutations to the human papillomaviruses type 16 sublineage A1 representative strain. Knowledge of the HPV 16 variant is fundamental, and these findings will allow the rational use of this animal model to explore the role of the A1 sublineage in HPV-driven cancer.pt_PT
dc.description.sponsorshipThis work is a result of Fundação para a Ciência e a Tecnologia (FCT); and also funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020); from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Por tugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT). This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundos Nacionais through the Fundação para a Ciência e a Tec nologia within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics); and from LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); 2SMART (NORTE-01-0145- FEDER-000054), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2022 Oct 15;23(20):12371. doi: 10.3390/ijms232012371pt_PT
dc.identifier.doi10.3390/ijms232012371pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/8481
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG e ITQB)
dc.relationiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG e ITQB)
dc.relationCentre for Toxicogenomics and Human Health
dc.relationLaboratory for Process Engineering, Environment, Biotechnology and Energy
dc.relationLaboratory for Process Engineering, Environment, Biotechnology and Energy
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/20/12371pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHuman Papillomaviruspt_PT
dc.subjectHPVpt_PT
dc.subjectHPV16pt_PT
dc.subjectK14HPV16pt_PT
dc.subjectCarcinogenesispt_PT
dc.subjectLineagept_PT
dc.subjectVariantpt_PT
dc.subjectInfecções Sexualmente Transmissíveispt_PT
dc.titleCharacterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG e ITQB)
oaire.awardTitleiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG e ITQB)
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleLaboratory for Process Engineering, Environment, Biotechnology and Energy
oaire.awardTitleLaboratory for Process Engineering, Environment, Biotechnology and Energy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04462%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04462%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F00009%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00511%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00511%2F2020/PT
oaire.citation.issue20pt_PT
oaire.citation.startPage12371pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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