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Browsing by Issue Date, starting with "2022-10-15"

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  • Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis
    Publication . Cochicho, Daniela; Nunes, Alexandra; Gomes, João Paulo; Martins, Luís; Cunha, Mário; Medeiros-Fonseca, Beatriz; Oliveira, Paula; Bastos, Margarida M.S.M.; Medeiros, Rui; Mendonça, Joana; Vieira, Luis; Gil da Costa, Rui M.; Felix, Ana
    The study of ()-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. This study aimed to determine the HPV16 variant and sublineage present in the K14HPV16 mouse model. This information can be considered of great importance to further enhance this K14HPV16 model as an essential research tool and optimize its use for basic and translational studies. Our study evaluated HPV DNA from 17 samples isolated from 4 animals, both wild-type (n = 2) and HPV16-transgenic mice (n = 2). Total DNA was extracted from tissues and the detection of HPV16 was performed using a qPCR multiplex. HPV16-positive samples were subsequently whole-genome sequenced by next-generation sequencing techniques. The phylogenetic positioning clearly shows K14HPV16 samples clustering together in the sub-lineage A1 (NC001526.4). A comparative genome analysis of K14HPV16 samples revealed three mutations to the human papillomaviruses type 16 sublineage A1 representative strain. Knowledge of the HPV 16 variant is fundamental, and these findings will allow the rational use of this animal model to explore the role of the A1 sublineage in HPV-driven cancer.
    2022-10-15Journal article Open access Show more
  • Multi-element and multi-isotopic profiles of Port and Douro wines as tracers for authenticity
    Publication . Coelho, Inês; Matos, Ana Sofia; Epova, Ekaterina N.; Barre, Julien; Cellier, Robin; Ogrinc, Nives; Castanheira, Isabel; Bordado, João; Donard, Olivier F.X.
    Port and Douro wines are produced in the Douro Demarcated Region using different wine making processes. This works aims at evaluating the influence of the geographical origin and the wine making process in their multielement (Li, Be, B, Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Mo, Ag, Cd, Sn, Tl, Pb, Fe, Mg, P, Ca, Na, K) and isotopic compositions (87Sr/86Sr, δ11B, xPb/yPb, δ18O). Fifteen samples of each were analysed. ANOVA identified significant differences for Ca, Na, Rb, Zn, Cu, Cr, Se, 206Pb/204Pb, 207Pb/204Pb, 206Pb/207Pb and δ11B. PCA revealed that Cr, Se and Cu are the main contributors for discrimination by type of wine. 87Sr/86Sr and δ18O are region specific and not influenced by the winemaking process or storage. Thus, make good natural tracers for geographical origin of this region. HCA suggested a common profile in Port and Douro in white wines, for Sr and K, which should be further investigated.
    2022-10-15Journal article Restricted access Show more