Publication
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
| dc.contributor.author | Ziane, Hanifa | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Bektache, Soumia | |
| dc.contributor.author | Tazir, Mohamed | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2016-03-24T13:03:51Z | |
| dc.date.available | 2020-12-01T01:30:10Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction. | pt_PT |
| dc.description.sponsorship | H. Ziane was supported by a grant from Ministère de l'Enseignement Superieur et de la Recherche Scientifique in Algeria. V. Manageiro was supported by the grant SFRH/BPD/77486/2011, from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal. | pt_PT |
| dc.identifier.citation | J Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4. | pt_PT |
| dc.identifier.doi | 10.1016/j.mimet.2015.11.002 | pt_PT |
| dc.identifier.issn | 0167-7012 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/3730 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0167701215301056 | pt_PT |
| dc.subject | Multiplex PCR | pt_PT |
| dc.subject | Pneumococci | pt_PT |
| dc.subject | Quellung Reaction | pt_PT |
| dc.subject | Sensitivity | pt_PT |
| dc.subject | Specificity | pt_PT |
| dc.subject | Serogroup/type | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 246 | pt_PT |
| oaire.citation.startPage | 243 | pt_PT |
| oaire.citation.title | Journal of Microbiological Methods | pt_PT |
| oaire.citation.volume | 119 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |