Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme

Ziane, Hanifa; Manageiro, Vera; Ferreira, Eugénia; Bektache, Soumia; Tazir, Mohamed; Caniça, Manuela

http://hdl.handle.net/10400.18/3730

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Abstract(s)

We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.