Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme

Ziane, Hanifa; Manageiro, Vera; Ferreira, Eugénia; Bektache, Soumia; Tazir, Mohamed; Caniça, Manuela

http://hdl.handle.net/10400.18/3730

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Resumo(s)

We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.

Palavras-chave

Multiplex PCR Pneumococci Quellung Reaction Sensitivity Specificity Serogroup/type Resistência aos Antimicrobianos

URI

http://hdl.handle.net/10400.18/3730

Citação

J Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4.

Editora

Elsevier

DOI

10.1016/j.mimet.2015.11.002

Coleções

DDI - Artigos em revistas internacionais

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