Repository logo
 
Publication

Antibody Binding and Complement-Mediated Killing of Invasive Haemophilus influenzae Isolates from Spain, Portugal, and the Netherlands

2020-10Journal article Open access
Simple item page
dc.contributor.authorDudukina, Elena
dc.contributor.authorde Smit, Laura
dc.contributor.authorVerhagen, Giel J.A.
dc.contributor.authorvan de Ende, Arie
dc.contributor.authorMarimón, José María
dc.contributor.authorBajanca-Lavado, Paula
dc.contributor.authorArdanuy, Carmen
dc.contributor.authorMarti, Sara
dc.contributor.authorde Jonge, Marien I.
dc.contributor.authorLangereis, Jeroen D.
dc.date.accessioned2021-04-02T15:38:00Z
dc.date.available2021-04-02T15:38:00Z
dc.date.issued2020-10
dc.description.abstractHaemophilus influenzae is a Gram-negative bacterium that can be classified into typeable (types a through f) and nontypeable (NTHi) groups. This opportunistic pathogen asymptomatically colonizes the mucosal epithelium of the upper respiratory tract, from where it spreads to other neighboring regions, potentially leading to disease. Infection with NTHi can cause otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and pneumonia, but it is increasingly causing invasive disease, including bacteremia and meningitis. Invasive NTHi strains are more resistant to complement-mediated killing. However, the mechanisms of complement resistance have never been studied in large numbers of invasive NTHi strains. In this study, we determined the relationship between binding of IgG or IgM and the bacterial survival in normal human serum for 267 invasive H. influenzae strains from Spain, Portugal, and the Netherlands, of which the majority (200 [75%]) were NTHi. NTHi bacteria opsonized with high levels of IgM had the lowest survival in human serum. IgM binding to the bacterial surface, but not IgG binding, was shown to be associated with complement-mediated killing of NTHi strains. We conclude that evasion of IgM binding by NTHi strains increases survival in blood, thereby potentially contributing to their ability to cause severe invasive diseases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInfect Immun. 2020 Oct;88(10):e00454-20. doi: 10.1128/IAI.00454-20. Print 2020 Sep 18.pt_PT
dc.identifier.doi10.1128/IAI.00454-20pt_PT
dc.identifier.issn0019-9567
dc.identifier.urihttp://hdl.handle.net/10400.18/7625
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttps://iai.asm.org/content/88/10/e00454-20.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHaemophilus influenzaept_PT
dc.subjectIgGpt_PT
dc.subjectIgMpt_PT
dc.subjectBacteremiapt_PT
dc.subjectComplement Evasionpt_PT
dc.subjectMeningitispt_PT
dc.subjectSepsispt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.titleAntibody Binding and Complement-Mediated Killing of Invasive Haemophilus influenzae Isolates from Spain, Portugal, and the Netherlandspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue10pt_PT
oaire.citation.startPagee00454-20pt_PT
oaire.citation.titleInfection and Immunitypt_PT
oaire.citation.volume88pt_PT
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
21_Antibody Binding and Complement.pdf
Size:
1.03 MB
Format:
Adobe Portable Document Format
Download

Collections

DDI - Artigos em revistas internacionais