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Putative Role of an ABC Efflux System in Aliarcobacter butzleri Resistance and Virulence

2023-02-06Journal article Open access
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dc.contributor.authorMartins, Inês
dc.contributor.authorMateus, Cristiana
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2024-01-12T14:29:28Z
dc.date.available2024-01-12T14:29:28Z
dc.date.issued2023-02-06
dc.description.abstractAbstract: Aliarcobacter butzleri is considered a ubiquitous microorganism and emergent pathogen, for which increasing rates of multidrug resistance have been described. In line with this, the present work aimed to evaluate for the first time the contribution of an ABC efflux system, the YbhFSR, in the resistance and virulence of this bacterium. Following the in silico characterization of the YbhFSR transporter, a mutant strain was constructed by inactivating the gene responsible for ATP-binding. After ensuring that the mutation did not have an impact on bacterial growth, the resistance profile of parental and mutant strains to different antimicrobial agents was evaluated. The results suggest that the efflux pump may influence the resistance to benzalkonium chloride, ethidium bromide, and cadmium, and several other compounds were identified as potential substrates. Regarding the evaluation of the accumulation of ethidium bromide, a slight increase was observed for the mutant strain, demonstrating a potential role of the YbhFSR efflux pump in the extrusion of toxic compounds from A. butzleri. Subsequently, the role of this efflux pump on the A. butzleri known virulence properties was evaluated, but no difference was seen among mutant and parental strains for the motility, biofilm formation ability, susceptibility to oxidative stress, or the ability to adhere and invade Caco-2 cells. However, in contrast to the parental strain, the mutant strain showed a resistance to human serum. Overall, the results support the role of efflux pumps in A. butzleri resistance to antimicrobials, highlighting the particular role of the YbhFSR system.pt_PT
dc.description.sponsorshipThis work was developed within the scope of the CICS-UBI projects UIDB/00709/2020 and UIDP/00709/2020, funded by national funds through the Portuguese Foundation for Science and Technology/MCTES.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2023 Feb 6;12(2):339. doi: 10.3390/antibiotics12020339pt_PT
dc.identifier.doi10.3390/antibiotics12020339pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.18/8895
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/12/2/339pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectABC Efflux Pumpspt_PT
dc.subjectAliarcobacter butzleript_PT
dc.subjectYbhFSRpt_PT
dc.subjectResistancept_PT
dc.subjectVirulencept_PT
dc.subjectGastrointestinal Infectionspt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titlePutative Role of an ABC Efflux System in Aliarcobacter butzleri Resistance and Virulencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.citation.issue2pt_PT
oaire.citation.startPage339pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublicatione0fbaa7c-391a-479d-9bcf-efb7997d4004
relation.isProjectOfPublication.latestForDiscoveryced21566-cfde-4640-afff-0536a07c1f8f

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