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The plasma membrane-enriched fraction proteome response during adaptation to hydrogen peroxide in Saccharomyces cerevisiae

2012-10Journal article Restricted access
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dc.contributor.authorPedroso, Nuno
dc.contributor.authorGomes Alves, Patricia
dc.contributor.authorMarinho, H. Susana
dc.contributor.authorBrito, Veronica
dc.contributor.authorBoada, Cristina
dc.contributor.authorAntunes, Fernando
dc.contributor.authorHerrero, Enrique
dc.contributor.authorPenque, Deborah
dc.contributor.authorCyrne, Luísa
dc.date.accessioned2013-02-12T12:27:54Z
dc.date.available2013-02-12T12:27:54Z
dc.date.issued2012-10
dc.description.abstractn Saccharomyces cerevisiae, adaptation to hydrogen peroxide (H₂O₂) decreases plasma membrane permeability to H₂O₂, changes its lipid composition and reorganizes ergosterol-rich microdomains by a still unknown mechanism. Here we show, by a quantitative analysis of the H₂O₂-induced adaptation effect on the S. cerevisiae plasma membrane-enriched fraction proteome, using two-dimensional gel electrophoresis, that 44 proteins are differentially expressed. Most of these proteins were regulated at a post-transcriptional level. Fourteen of these proteins contain redox-sensitive cysteine residues and nine proteins are associated with lipid and vesicle traffic. In particular, three proteins found in eisosomes and in the eisosome-associated membrane compartment occupied by Can1p were up-regulated (Pil1p, Rfs1p and Pst2p) during adaptation to H₂O₂. Survival studies after exposure to lethal H₂O₂ doses using yeast strains bearing a gene deletion corresponding to proteins associated to lipid and vesicle traffic demonstrated for the first time that down-regulation of Kes1p, Vps4p and Ynl010wp and up-regulation of Atp1 and Atp2 increases resistance to H₂O₂. Moreover, for the pil1Δ strain, H₂O₂ at low levels produces a hormetic effect by increasing proliferation. In conclusion, these data further confirms the plasma membrane as an active cellular site during adaptation to H₂O₂ and shows that proteins involved in lipid and vesicle traffic are important mediators of H₂O₂ adaptation.por
dc.identifier.citationFree Radic Res. 2012 Oct;46(10):1267-79. Epub 2012 Jul 16por
dc.identifier.issn1071-5762
dc.identifier.otherdoi: 10.3109/10715762.2012.704997
dc.identifier.urihttp://hdl.handle.net/10400.18/1283
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInforma Healthcarepor
dc.relation.publisherversionhttp://informahealthcare.com/doi/abs/10.3109/10715762.2012.704997por
dc.subjectProteomicspor
dc.subjectGenómica Funcional e Estruturalpor
dc.subjectEisosomespor
dc.subjectMembrane Compartment of Can1ppor
dc.subjectOxidative Stresspor
dc.subjectPlasma Membrane Permeabilitypor
dc.subjectLipid Trafficpor
dc.titleThe plasma membrane-enriched fraction proteome response during adaptation to hydrogen peroxide in Saccharomyces cerevisiaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1279por
oaire.citation.startPage1267por
oaire.citation.titleFree Radical Researchpor
oaire.citation.volume46(10)por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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