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Introduction to hCOMET special issue, ‘Comet assay in vitro’
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Orientador(es)
Resumo(s)
This special issue is produced as a valuable outcome of the COST
Action hCOMET. A major aim of this project is to encourage the
adoption of standard procedures for the comet assay, in order to improve
reliability. Comet assay procedures tend to vary from one laboratory
to another, and so comparing results between laboratories can
be problematic. Much work has been done to establish how different
parameters, such as agarose concentration, lysis time, or electrophoresis
voltage gradient can affect results, and an aspiration of hCOMET is
to devise standard procedures. In addition to papers on practical issues
relating to the use of the comet assay, there are several descriptions of
novel model cell systems. Finally, there are accounts of various applications
of the assay to in vitro genotoxicity testing (mainly on human
cell lines), as well as investigations of antigenotoxicity. (...)
Descrição
Editorial
Palavras-chave
Animals Biological Monitoring Cell Line Comet Assay Humans Laboratory Proficiency Testing Reproducibility of Results
Contexto Educativo
Citação
Mutat Res. 2019 Sep;845:403071. doi: 10.1016/j.mrgentox.2019.07.001. Epub 2019 Jul 3
Editora
Elsevier