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Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

2020-04-27Journal article Open access
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dc.contributor.authorSabença, Carolina
dc.contributor.authorde Sousa, Telma
dc.contributor.authorOliveira, Soraia
dc.contributor.authorViala, Didier
dc.contributor.authorThéron, Laetitia
dc.contributor.authorChambon, Christophe
dc.contributor.authorHébraud, Michel
dc.contributor.authorBeyrouthy, Racha
dc.contributor.authorBonnet, Richard
dc.contributor.authorCaniça, Manuela
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.date.accessioned2021-04-02T16:21:04Z
dc.date.available2021-04-02T16:21:04Z
dc.date.issued2020-04-27
dc.description.abstractVancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6')-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 ± 3, and a peak at m/z 6048 ± 1 allowed us to distinguish Enterococcusfaecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.pt_PT
dc.description.sponsorshipThis work was supported by the “Espectrometria de massa e sequenciação aplicadas à microbiologia clínica moderna: pesquisa e identificação de biomarcadores em espécies bacterianas multiresistentes” (Programa de Acções Universitárias Integradas Luso-Francesas, Ação n.º: TC-14 / 2017) and by the Associate Laboratory for Green Chemistry, LAQV, which is financed by national funds from FCT/MCTES (UID/QUI/50006/2020). The authors are grateful for the support of UIDB/00211/2020 funded by FCT/MCTES through national funds. We also thank the Ministere de l’Education Nationale, de l’Enseignement Superieur et de la Recherche, INSERM (UMR1071), INRA (USC-2018), Santé Publique France, and the Centre Hospitalier Regional Universitaire de Clermont-Ferrand, France.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiology (Basel). 2020 Apr 27;9(5):89. doi: 10.3390/biology9050089.pt_PT
dc.identifier.doi10.3390/biology9050089pt_PT
dc.identifier.issn2079-7737
dc.identifier.urihttp://hdl.handle.net/10400.18/7628
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/QUI/50006/2020pt_PT
dc.relationCenter for the Study of Animal Science
dc.relation.publisherversionhttps://www.mdpi.com/2079-7737/9/5/89pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEnterococcus spppt_PT
dc.subjectMALDI-TOF MSpt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectNext-generation Sequencingpt_PT
dc.subjectProcessed Meatpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleNext-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCenter for the Study of Animal Science
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00211%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage89pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7
relation.isProjectOfPublication.latestForDiscovery69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7

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