Browsing by Issue Date, starting with "2020-04-27"
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- Efeitos genéticos e epigenéticos da exposição a nanomateriaisPublication . Ventura, Célia; Louro, Henriqueta; Silva, Maria JoãoAula 7: Efeitos genéticos e epigenéticos da exposição a nanomateriais. Efeitos genotóxicos dos nanomateriais; análise da genotoxicidade de um nanotubo de carbono (NTC); Toxicogenómica, transcriptómica e epigenómica; Transcriptómica; estudo global da expressão génica após exposição in vivo a um NTC; Epigenómica: alterações nas histonas, metilação do DNA e expressão de microRNAs; análise da expressão diferencial de microRNAs após exposição in vitro a um NTC; Conclusões
- Nanomateriais - o que são, que riscos comportamPublication . Pinto, Fátima; Louro, Henriqueta; Silva, Maria JoãoAula 6: Nanomateriais - o que são, que riscos comportam. Nanomaterials (NM) – Materials with one or more components that have at least one dimension in the range of 1 to 100 nm
- O projeto HBM4EU - exemplo de biomonitorização da exposição ocupacional ao crómioPublication . Tavares, Ana Maria; Louro, Henriqueta; Silva, Maria JoãoAula 5: O projeto HBM4EU - exemplo de biomonitorização da exposição ocupacional ao crómio. Projeto HBM4EU - General objective: To understand human exposure to chemicals and resulting health impacts and communicate with policy makers to ensure that the results are exploited in the design of new chemicals policies and the evaluation of existing measures.
- Toxicologia Genética - Vias de Efeitos Adversos e Avaliação de RiscoPublication . Rolo, Dora; Silva, Maria João; Louro, HenriquetaAula sobre Vias de Efeitos Adversos e Avaliação de Risco
- Nanomateriais ingeridos: efeitos celulares e genéticosPublication . Vital, Nádia; Silva, Maria João; Louro, HenriquetaAula sobre os efeitos celulares e genéticos dos nanomateriais ingeridos.
- Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)Publication . Sabença, Carolina; de Sousa, Telma; Oliveira, Soraia; Viala, Didier; Théron, Laetitia; Chambon, Christophe; Hébraud, Michel; Beyrouthy, Racha; Bonnet, Richard; Caniça, Manuela; Poeta, Patrícia; Igrejas, GilbertoVancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6')-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 ± 3, and a peak at m/z 6048 ± 1 allowed us to distinguish Enterococcusfaecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.