Publication
Virulence and antibiotic resistance plasticity of Arcobacter butzleri: Insights on the genomic diversity of an emerging human pathogen
| dc.contributor.author | Isidro, Joana | |
| dc.contributor.author | Ferreira, Susana | |
| dc.contributor.author | Pinto, Miguel | |
| dc.contributor.author | Domingues, Fernanda | |
| dc.contributor.author | Oleastro, Mónica | |
| dc.contributor.author | Gomes, João Paulo | |
| dc.contributor.author | Borges, Vítor | |
| dc.date.accessioned | 2020-04-30T22:15:28Z | |
| dc.date.available | 2020-04-30T22:15:28Z | |
| dc.date.issued | 2020-06 | |
| dc.description.abstract | Arcobacter butzleri is a foodborne emerging human pathogen, frequently displaying a multidrug resistant character. Still, the lack of comprehensive genome-scale comparative analysis has limited our knowledge on A. butzleri diversification and pathogenicity. Here, we performed a deep genome analysis of A. butzleri focused on decoding its core- and pan-genome diversity and specific genetic traits underlying its pathogenic potential and diverse ecology. A. butzleri (genome size 2.07-2.58 Mbp) revealed a large open pan-genome with 7474 genes (about 50% being singletons) and a small but diverse core-genome with 1165 genes. It presents a plastic virulome (including newly identified determinants), marked by the differential presence of multiple adaptation-related virulence factors, such as the urease cluster ureD(AB)CEFG (phenotypically confirmed), the hypervariable hemagglutinin-encoding hecA, a type I secretion system (T1SS) harboring another agglutinin and a novel VirB/D4 T4SS likely linked to interbacterial competition and cytotoxicity. In addition, A. butzleri harbors a large repertoire of efflux pumps (EPs) and other antibiotic resistant determinants. We unprecedentedly describe a genetic mechanism of A. butzleri macrolides resistance, (inactivation of a TetR repressor likely regulating an EP). Fluoroquinolones resistance correlated with Thr-85-Ile in GyrA and ampicillin resistance was linked to an OXA-15-like β-lactamase. Remarkably, by decoding the polymorphism pattern of the main antigen PorA, we show that A. butzleri is able to exchange porA as a whole and/or hypervariable epitope-encoding regions separately, leading to a multitude of chimeric PorA presentations that can impact pathogen-host interaction during infection. Ultimately, our unprecedented screening of short sequence repeats indicates that phase variation likely modulates A. butzleri key adaptive functions. In summary, this study constitutes a turning point on A. butzleri comparative genomics revealing that this human gastrointestinal pathogen is equipped with vast and diverse virulence and antibiotic resistance arsenals that open a multitude of phenotypic fingerprints for environmental/host adaptation and pathogenicity. | pt_PT |
| dc.description.sponsorship | This work was partially supported by funds from the Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior (CICS-UBI), through National Funds by FCT - Foundation for Science and Technology (UID/Multi/00709/2019), Portugal. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Infect Genet Evol. 2020 Jun;80:104213. doi: 10.1016/j.meegid.2020.104213. Epub 2020 Jan 30 | pt_PT |
| dc.identifier.doi | 10.1016/j.meegid.2020.104213 | pt_PT |
| dc.identifier.issn | 1567-1348, | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6570 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/abs/pii/S1567134820300459?via%3Dihub | pt_PT |
| dc.subject | Antibiotic Resistance | pt_PT |
| dc.subject | Arcobacter butzleri | pt_PT |
| dc.subject | Genome Diversity | pt_PT |
| dc.subject | Phase Variation | pt_PT |
| dc.subject | Virulence Factors | pt_PT |
| dc.subject | porA | pt_PT |
| dc.subject | Infecções Gastrointestinais | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Virulence and antibiotic resistance plasticity of Arcobacter butzleri: Insights on the genomic diversity of an emerging human pathogen | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT | |
| oaire.citation.startPage | 104213 | pt_PT |
| oaire.citation.title | Infection, Genetics and Evolution | pt_PT |
| oaire.citation.volume | 80 | pt_PT |
| oaire.fundingStream | 5876 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.embargofct | De acordo com política editorial da revista. | pt_PT |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | f5e75317-d96b-4bfe-97b5-3912e4125430 | |
| relation.isProjectOfPublication.latestForDiscovery | f5e75317-d96b-4bfe-97b5-3912e4125430 |