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PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal

2016-11Journal article Restricted access
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dc.contributor.authorSantos-Silva, M.M.
dc.contributor.authorMelo, P.
dc.contributor.authorSantos, N.
dc.contributor.authorAntunes, S.
dc.contributor.authorDuarte, L.R.
dc.contributor.authorFerrolho, J.
dc.contributor.authorMilhano, N.
dc.contributor.authorSantos, P.T.
dc.contributor.authorDomingos, A.
dc.contributor.authorSantos, A.S.
dc.date.accessioned2017-03-03T16:18:56Z
dc.date.available2021-11-26T01:30:10Z
dc.date.issued2016-11
dc.description.abstractThe Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs). This study presents the results of TBPs survey on ticks collected at sensitive conservation areas of Southeast Portugal. From 2012 to 2014, 231 ticks obtained from vegetation, sympatric domestic and wild animals were submitted for analysis. The presence of Babesia spp., Cytauxzoon spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., Candidatus Neoehrlichia mikurensis, among other Anaplasmataceae, and Coxiella burnetii were investigated by PCR. Six tick species were recorded, Dermacentor marginatus (n = 13/5.6%), Hyalomma lusitanicum (n = 175/75.8%), Ixodes ricinus (n = 4/1.7%), Rhipicephalus bursa (n = 7/3.0%), R. pusillus (n = 21/9.1%) and R. sanguineus sensu lato (n = 11/4.8%). The molecular screening confirmed the presence of two tick-borne pathogens, C. burnetii (N = 34) and Anaplasma platys (N = 1), and one tick-endosymbiont, Candidatus Midichloria mitochondrii (N = 45). The results obtained provide new information on the circulation of ticks and TBPs with potential veterinary importance in Iberian lynx habitat.pt_PT
dc.description.sponsorshipThis research was supported by Programa LIFE-Natureza da Comissão Europeia [project reference LIFE08NATP000227] and the Fundação para a Ciencia e Tecnologia [projects references PTDC/SAU-SAP/115266/2009, PTDC/CVT-EPI/4339/2012 and GHTMeUID/Multi/04413/2013]. Hepatozoon canis DNA control was kindly provided by Carla Maia DVM PhD, Instituto de Higiene e Medicina Tropical (IHMT).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMol Cell Probes. 2017 Feb;31:42-45. doi: 10.1016/j.mcp.2016.11.005. Epub 2016 Nov 25pt_PT
dc.identifier.doi10.1016/j.mcp.2016.11.005pt_PT
dc.identifier.issn0890-8508
dc.identifier.urihttp://hdl.handle.net/10400.18/4456
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationQ fever - from diagnosis to eco-epidemiological investigation of Coxiella burnetii in the context of human infection
dc.relationFunctional genomics of Rhipicephalus bursa and Babesia ovis interactions towards disease control.
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0890850816301153pt_PT
dc.subjectAnaplasma Platyspt_PT
dc.subjectCandidatus Midichloria Mitochondriipt_PT
dc.subjectCoxiella Burnetiipt_PT
dc.subjectHyalomma Lusitanicumpt_PT
dc.subjectIberian Lynx Habitatpt_PT
dc.subjectTick-borne Agentspt_PT
dc.subjectTickspt_PT
dc.subjectSoutheast Portugalpt_PT
dc.subjectInfecções Sistémicas e Zoonoses
dc.titlePCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleQ fever - from diagnosis to eco-epidemiological investigation of Coxiella burnetii in the context of human infection
oaire.awardTitleFunctional genomics of Rhipicephalus bursa and Babesia ovis interactions towards disease control.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-SAP%2F115266%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-EPI%2F4339%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04413%2F2013/PT
oaire.citation.endPage45pt_PT
oaire.citation.startPage42pt_PT
oaire.citation.titleMolecular and Cellular Probespt_PT
oaire.citation.volume31pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication855b2602-1f42-4d60-ab76-44210d556ab6
relation.isProjectOfPublication3f4b6e4b-5437-4cee-8a5b-03cc9497c3f4
relation.isProjectOfPublicationad39241e-5511-4aa7-975d-680cd5082c36
relation.isProjectOfPublication.latestForDiscovery3f4b6e4b-5437-4cee-8a5b-03cc9497c3f4

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