Publication
MicroRNA expression changes in A549 alveolar epithelial cells after 7 days in vitro incubation
| dc.contributor.author | Ventura, Célia | |
| dc.contributor.author | Vieira, Luís | |
| dc.contributor.author | Silva, Catarina | |
| dc.contributor.author | Sousa-Uva, António | |
| dc.contributor.author | Silva, Maria João | |
| dc.date.accessioned | 2019-02-20T10:56:04Z | |
| dc.date.available | 2019-02-20T10:56:04Z | |
| dc.date.issued | 2018-10 | |
| dc.description.abstract | Introduction: A549 human alveolar type II epithelial cells have been widely used to study airway physiology and pathology, drug metabolism, effects of pulmonary exposure to viruses and toxicants and in cancer research. Because these studies are mainly based on long-term cultures to mimic real life exposure, it is pertinent to consider whether intrinsic cellular changes may occur in A549 cells over time. To this end, microRNA (miRNA) differential expression (DE) profiling may be used, since it provides a global view of cellular molecular pathways modified in response to external signals. Objectives: In this study, we used next-generation sequencing (NGS) to identify differently expressed miRNAs in viable A549 cells cultured for 7 days without manipulation comparatively to 24 h cultures, and the molecular and biological pathways that were altered. | pt_PT |
| dc.description.sponsorship | This work was co-funded by the Foundation for Science and Technology through the ToxOmics (UID/BIM/00009/2013) and ToxApp4NanoCELFI (PTDC/SAU-PUB/ 32587/2017) | pt_PT |
| dc.description.version | N/A | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.18/5913 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | A549 Cells | pt_PT |
| dc.subject | microRNA | pt_PT |
| dc.subject | Epigenomics | pt_PT |
| dc.subject | Molecular Pathways | pt_PT |
| dc.subject | Genotoxicidade Ambiental | pt_PT |
| dc.title | MicroRNA expression changes in A549 alveolar epithelial cells after 7 days in vitro incubation | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT | |
| oaire.citation.conferencePlace | Berlim, Alemanha | pt_PT |
| oaire.citation.title | 20th International Congress on In Vitro Toxicology (ESTIV2018) - New approach methodologies for in vitro toxicology applications, 15-18 October 2018 | pt_PT |
| oaire.fundingStream | 5876 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isProjectOfPublication | e9cc9728-4f09-4e3a-b30d-53d4429986fb | |
| relation.isProjectOfPublication.latestForDiscovery | e9cc9728-4f09-4e3a-b30d-53d4429986fb |