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Epigenomics as a novel approach to explore the toxic effects of nanomaterials

2023-12-01Lecture Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorVieira, Luís
dc.contributor.authorValente, Ana
dc.contributor.authorFernandes, Camila
dc.contributor.authorSilva, Catarina
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorSilva, Maria João
dc.date.accessioned2024-02-09T13:15:50Z
dc.date.available2024-02-09T13:15:50Z
dc.date.issued2023-12-01
dc.description.abstractIn recent years, there has been a huge development of innovative engineered nanomaterials with potential use in industrial and biomedical applications. This increased widespread use raised concerns that nanomaterials may elicit human adverse health effects through occupational, environmental or consumer exposure. Many toxicity studies, mainly in vitro, have showed that some nanomaterials, such as carbon nanotubes or titanium dioxide nanoparticles (TiO 2 NP), may cause genotoxicity, inflammation, and associated adverse health effects. Nevertheless, few studies have focused on the nanomaterials effect s on the epigenome, namely, modifications of histone tails, microRNA expression or DNA methylation. Here we wil l present two “omics” studies based on next generation sequencing , one focusing on the effect of three nanocellulose s derived from Eucaliptus globulus kraft pulp on the microRNA expression of BEAS 2B, and an other one focusing on the effect of three types of TiO 2 NP on the DNA methylation of Caco 2 cells. Regarding the former 24h exposure to fibrillar micro/nanocellulose s did not induced significant (FDR ≤ 0.05) differentially expressed microRNAs, as compared to non exposed cells. By contrast, the crystalline nanocelul l ose induced the over and under expression of 22 and 30 microRNAs, respectively. These microRNAs can be f urther explored as potential biomarkers for human biomonitoring and co ntribute to elucidate the mechanisms of action of crystalline nanocellulose. As to the genome wide methylation study Reduced Representative Bisulfite Sequencing allowed the identification of significant ( p ≤ 0.05) differential methylation of 92, 70, and 88 gene sequences for the anatase, rutile and brookite phase TiO 2 NP exposures, respectively. Functional pathway analysis of these methylation changes showed that all TiO 2 NP may affect cell proliferation, differentiation, and survival, and suggested different molecular mechanisms of action for each type of TiO 2 NP. In conclusion, epigenomics revealed to be a powerful tool to understand the key molecular events underlying nanomaterials effects.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT/ through national funds ( under the project ToxApp 4 NanoCELFI (PTDC/SAU PUB/ 32587 2017 project Nanoingest PTDC/SAU PUB/ 29481 2017 and projects UIDB/ 00009 2020 and UIDP/ 0009 2020 ToxOmics)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/9070
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectNanomaterialspt_PT
dc.subjectToxicoepigenomicspt_PT
dc.subjectMicroRNAspt_PT
dc.subjectMethylationpt_PT
dc.subjectFunctional Pathwayspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleEpigenomics as a novel approach to explore the toxic effects of nanomaterialspt_PT
dc.typelecture
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlaceValencia, Spainpt_PT
oaire.citation.titleNetworking on food safety and innovative techniques for evaluating human health risks: Workshop - RiskTox, 1 December 2023pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameLouro
person.familyNameSilva
person.givenNameCélia
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier2056460
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typelecturept_PT
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