Publication
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Romão, Raquel | |
| dc.contributor.author | Moura, Inês Barata | |
| dc.contributor.author | Sampaio, Daniel A. | |
| dc.contributor.author | Vieira, Luís | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2019-03-27T13:14:54Z | |
| dc.date.available | 2019-03-27T13:14:54Z | |
| dc.date.issued | 2018-11-27 | |
| dc.description | Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/ | pt_PT |
| dc.description.abstract | In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010-2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC-type (including one new variant: blaKPC-21), 1 blaGES-5, and 1 blaGES-6 plus blaKPC-3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC-21 in a 12,748 bp length plasmid. The blaKPC-21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates. | pt_PT |
| dc.description.sponsorship | VM was supported by Fundação para a Ciência e a Tecnologia (FCT) fellowship (Grant SFRH/BPD/ 77486/2011), financed by the European Social Funds (COMPETE-FEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN). The authors thank FCT for Project grant UID/MULTI/00211/2013. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Front Microbiol. 2018 Nov 27;9:2834. doi: 10.3389/fmicb.2018.02834. eCollection 2018. | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2018.02834 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6330 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation | EVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE | |
| dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fmicb.2018.02834/full | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Escherichia coli | pt_PT |
| dc.subject | EuSCAPE | pt_PT |
| dc.subject | KPC-21 | pt_PT |
| dc.subject | Klebsiella pneumoniae | pt_PT |
| dc.subject | Portugal | pt_PT |
| dc.subject | Carbapenemase-producing Enterobacteriaceae | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | EVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F77486%2F2011/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00211%2F2013/PT | |
| oaire.citation.startPage | 2834 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| oaire.fundingStream | 5876 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 3b63e7d9-d684-42ae-aff9-36815c617501 | |
| relation.isProjectOfPublication | 4641127c-7dd5-44f4-a3d1-d3c4d744ba51 | |
| relation.isProjectOfPublication.latestForDiscovery | 4641127c-7dd5-44f4-a3d1-d3c4d744ba51 |