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Assessment of benzoquinones and mycotoxins in cereal flour samples from Portugal within SafeGrains project
Publication . Alvito, Paula; Hilário, C; Barros, G; Duarte, S; Pires, M.J.; Magro, A; Assunção, R.; Ferreira, RB; Carvalho, MO
One of the most relevant problems in stored grain is the contamination by insects and mycotoxin-producing fungi. The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera, Tenebrionidae), one of the major key-pests of milled grain, is resistant to Beauveria bassiana infection, producing benzoquinone-containing defensive secretions, that reduce fungal germination and growth. It can be affirmed that the tenebrionid cuticular secretions have antifungal properties. For T. castaneum, p-benzoquinones such as methyl-1,4-benzoquinone (MBQ) and ethyl-1,4-benzoquinone (EBQ) are major components of defensive secretions used as repellents and irritants. SafeGrains project (PTDC/ASP‐PLA/28350/2017) intends to contribute to clarify the interaction among pest infestation and associated benzoquinones and fungus and produced mycotoxins. The present study aims to implement and validate two HPLC methodologies to determine benzoquinones and mycotoxins in cereal flour samples. In a first attempt, HPLC methods will be optimized and validated for cereal flour samples (linearity and working range, limits of detection (LOD) and quantification (LOQ) as well as recovery). Once attained the satisfactory analytical conditions for these determinations, the presence of both chemicals will be quantified in 5 flour samples from Portugal, contaminated with insects (T. castaneum) or fungi (mycotoxin producer as Aspergillus sp) or both. Results will be presented and discussed concerning its contents in benzoquinones and mycotoxins and possible interactive effects.
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Fundação para a Ciência e a Tecnologia

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5876

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UID/AGR/04129/2013

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