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- Resolvin E1-Chemerin receptor 1 axis is dysregulated in critical COVID-19 patientsPublication . Silva-Pereira, Carolina; Reina-Couto, Marta; Pereira-Terra, Patrícia; Teixeira-Santos, Luísa; Martins, Sandra; Pinho, Dora; Soares, Miguel Luz; Dias, Cláudia Camila; Sarmento, António; Tavares, Margarida; Guimarães, João Tiago; Paiva, José-Artur; Fraga, Sónia; Albino-Teixeira, António; Roncon-Albuquerque, Roberto; Sousa, TeresaResolvin E1 (RvE1) and Resolvin D1 (RvD1) are key resolvins implicated in inflammation resolution of respiratory and infectious diseases. In contrast to cytokines, they have been scarcely explored in COVID-19 and their ability for discriminating COVID-19 severity and patient outcomes has not been compared with that of cytokines. Therefore, among a panel comprising cytokines (interleukin (IL)-1beta, IL-6, IL-10, tumor necrosis factor alpha, interferon gamma and granulocyte-macrophage colony-stimulating factor), RvD1 and RvE1 and their respective receptors (FPR2, Chemerin1), we evaluated which mediators better distinguished COVID-19 severity, the need of mechanical ventilation and patient mortality. Blood was collected from 61 patients with “severe” (n = 27), “critical” (n = 17) and “critical on veno-venous extracorporeal membrane oxygenation (VV-ECMO)” (n = 17) COVID-19 at admission, days 3–4 and days 5–8, and from controls (n = 23) at a single time point. We measured cytokines by multiplex immunoassays, resolvins by enzyme-linked immunosorbent assays, and FPR2 and Chemerin1 mRNA by real-time quantitative polymerase chain reaction. We obtained principal component analysis (PCA)/partial least squares discriminant analysis (PLS-DA) models significantly differentiating (P < 0.001): controls from each patient group; “severe” from all critical patients; patients without or with mechanical ventilation, and survivors from non-survivors. RvE1 consistently showed a variable importance in projection (VIP) score > 2.5 and a p(corr) >0.8, being the most relevant discriminating variable. Univariate and repeated measures multivariate analyses showed higher RvE1 in “critical on VV-ECMO”, mechanically ventilated patients and non-survivors, while Chemerin1 exhibited an opposite profile. RvE1 positively correlated with inflammation and partial pressure of CO2, whereas Chemerin1 correlated with lower inflammation, better respiratory function and lower hospital length of stay. We conclude that RvE1 was the mediator best distinguishing COVID-19 severity and that RvE1-Chemerin1 axis is dysregulated in this disease.
- REVIVE - Leishmania infantum detected in Sergentomyia minuta in the Algarve region, PortugalPublication . Vilares, Anabela; Amaro, Fátima; Reis, Tânia; Martins, Susana; Alves, Maria João; Gargaté, Maria João; REVIVE TeamLeishmaniasis caused by Leishmania donovani represents a major public health concern in Mediterranean countries, with phlebotomine sand flies serving as the primary vectors for the transmission of Leishmania parasites [1]. In Portugal, REVIVE is a national surveillance network operating under the Ministry of Health since 2008, initially targeting mosquitoes, with its scope expanded to include ticks in 2011 and sand flies in 2016. The primary objectives of REVIVE are to identify the existing species in Portugal, establish which pathogens are circulating among them and provide alerts for prevention and control measures when necessary.
- Leishmaniasis retrospective analysis of cases diagnosed in the National Institute of Health between 2020 and 2024Publication . Reis, Tânia; Vilares, Anabela; Ferreira, Idalina; Martins, Susana; Gargaté, Maria JoãoAim: To describe the demographic characteristics of leishmaniasis cases, in patients with clinical symptoms, whose laboratory diagnosis was confirmed between 2020 and 2024 at the National Reference Lab of Parasitic and Fungal Infections.