Percorrer por data de Publicação, começado por "2025-06-17"
A mostrar 1 - 3 de 3
Resultados por página
Opções de ordenação
- Loss of BCL6 transcriptional repressor impacts migration but not viability in MCF-7 breast cancer cellsPublication . Dyson-Jorge, João; Jordan, Peter; Barros, Patrícia; Matos, PauloBreast cancer (BC) incidence has risen over the past two decades, now being the most prevalent cancer worldwide and the second leading cause of cancer-related deaths. Despite advancements in BC treatment, challenges like acquired resistance, recurrence, and metastasis persist. BCL6, a transcriptional repressor, acts as an oncogene in BC, being downregulated in about half of primary tumors of all BC subtypes and associated with disease progression and poor patient prognosis. This underscores the need to better understand BCL6 role in BC development. Therefore, we used RNA interference to explore the impact of BCL6 depletion on the oncogenic progression of MCF-7 cells, a low-tumorigenic estrogen receptor-positive cell line. While BCL6 is known to regulate normal mammary cell proliferation and differentiation, its depletion did not affect MCF-7 cell proliferation or viability but significantly reduced their individual and collective migratory properties. RNA microarray analysis identified a set of genes upregulated following BCL6 depletion, including S100A7, previously reported to inhibit MCF-7 cell migration and invasion by reducing MMP9 secretion. However, our findings showed that S100A7 downregulation alone did not affect MCF-7 migration. Moreover, simultaneous depletion of BCL6 and S100A7 failed to restore MCF-7 cell migratory behavior.
- Tolerance of microorganisms to surface disinfection in food productionPublication . Peixoto, Andreia; Santos, Susana; Rodrigues, João; Saraiva, Margarida; Belo Correia, Cristina; Batista, Rita; Novais, Carla; Antunes, PatríciaFood safety has a direct impact on consumer protection and public health,with food industry companies being primarily responsible for the safety of food products. Operators of food service businesses must ensure the preparation and distribution of safe meals by preventing, eliminating, or minimising microbiological risks at all stages. Contamination can occur at the final stage of food preparation if proper hygiene standards, such as cleaning and disinfecting surfaces,are not maintained. The persistence of bacterial hazards in the food preparation environment influences the occurrence of cross-contamination, compromising food safety. Despite recommended biocide use, poor hygiene of surfaces in direct or indirect contact with food was observed for most of the foodservice facilities studied. The presence of several recurrent bacterial species suggests possible persistence, reintroduction, and/or cross-contamination events. Investigating biocide susceptibility and bacterial persistence is essential to improve hygiene practices and identify more reliable bioindicators.
- Portugal - Developments on the Safety of Manufactured Nanomaterials and Advanced MaterialsPublication . Louro, HenriquetaItem 4. Developments in Member countries relevant to the WPMN Programme of Work: Tour de Table (ENV/CBC/NANO(2025). Delegations will be invited to highlight past or planned activities at the national level that are most relevant for the discussion of the WPMN Programme of Work, and for the work of the various steering groups. An updated document will be prepared after the WPMN.