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- Multi-country and intersectoral assessment of cluster congruence between pipelines for genomics surveillance of foodborne pathogensPublication . Mixão, Verónica; Pinto, Miguel; Brendebach, Holger; Sobral, Daniel; Santos, João Dourado; Radomski, Nicolas; Uldall, Anne Sophie Majgaard; Bomba, Arkadiusz; Pietsch, Michael; Bucciacchio, Andrea; de Ruvo, Andrea; Castelli, Pierluigi; Iwan, Ewelina; Simon, Sandra; Coipan, Claudia E.; Linde, Jörg; Petrovska, Liljana; Kaas, Rolf Sommer; Joensen, Katrine Grimstrup; Nielsen, Sofie Holtsmark; Kiil, Kristoffer; Lagesen, Karin; Di Pasquale, Adriano; Gomes, João Paulo; Deneke, Carlus; Tausch, Simon H.; Borges, VítorDifferent laboratories employ different Whole-Genome Sequencing (WGS) pipelines for Food and Waterborne disease (FWD) surveillance, casting doubt on the comparability of their results and hindering optimal communication at intersectoral and international levels. Through a collaborative effort involving eleven European institutes spanning the food, animal, and human health sectors, we aimed to assess the inter-pipeline clustering congruence across all resolution levels and perform an in-depth comparative analysis of cluster composition at outbreak level for four important foodborne pathogens: Listeria monocytogenes, Salmonella enterica, Escherichia coli, and Campylobacter jejuni. We found a general concordance between allele-based pipelines for all species, except for C. jejuni, where the different resolution power of allele-based schemas led to marked discrepancies. Still, we identified non-negligible differences in outbreak detection and demonstrated how a threshold flexibilization favors the detection of similar outbreak signals by different laboratories. These results, together with the observation that different traditional typing groups (e.g., serotypes) exhibit a remarkably different genetic diversity, represent valuable information for future outbreak case-definitions and WGS-based nomenclature design. This study reinforces the need, while demonstrating the feasibility, of conducting continuous pipeline comparability assessments, and opens good perspectives for a smoother international and intersectoral cooperation towards an efficient One Health FWD surveillance.