Browsing by Issue Date, starting with "2016-07-09"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- The contribution of the European high containment laboratories during the 2014-2015 Ebola Virus Disease emergencyPublication . Nisii, C.; Vincenti, D.; Fusco, F.M.; Schmidt-Chanasit, J.; Carbonnelle, C.; Raoul, H.; Eickmann, M.; Hewson, R.; Brave, A.; Nuncio, M.S.; Sanchez-Seco, M.P.; Palyi, B.; Kis, Z.; Zange, S.; Nitsche, A.; Kurth, A.; Strasser, M.; Capobianchi, M.R.; Ozin, A.; Guglielmetti, P.; Menel-Lemos, C.; Jacob, D.; Grunow, R.; Ippolito, G.; Di Caro, A.Since December 2013, the world has experienced the worst ever epidemic of Ebola virus disease (EVD), which has caused thousands of deaths in several West African countries. When the epidemic began, the European Union (EU) was not unprepared, thanks to the 10-year-long commitment of the European Commission (EC) to fund several networks in the area of highly infectious diseases. The European Network of Biosafety-Level 4 (BSL-4) laboratories (Euronet-P4, later called ENP4-Lab) was one of them; it has been operating since 2004, bringing together the facilities where RiskGroup 4 (RG-4) pathogens such as Ebola virus can be safely handled. In 2010, with the aim of increasing European preparedness in the fight against highly infectious trans-border threats, a new Joint Action was launched, resulting from the union of the networks that had previously worked on the diagnostics of highly infectious viruses and bacteria: ENP4-Lab and EQADeBa (Establishment of Quality Assurance for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk). (...)
- Anaplasma marginale and Theileria annulata in questing ticks from PortugalPublication . Antunes, S.; Ferrolho, J.; Domingues, N.; Santos, A.S.; Santos-Silva, M.M.; Domingos, A.Ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. Monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. In this study 428 host-seeking ticks, belonging to nine species of Ixodidae and collected from 17 of the 23 Portuguese mainland subregions, were screened for several tick-borne agents with veterinary relevance: Anaplasma marginale, Anaplasma ovis, Anaplasma centrale, Babesia spp., Coxiella burnetii and Theileria spp. Prevalence was assessed by PCR and amplified amplicons sequenced for validation of results. Twenty ticks, in a total of 428, were found positive: one Ixodes ventalloi for Theileria annulata and four Dermacentor marginatus, one Haemaphysalis punctata, five Ixodes ricinus, five I. ventalloi, and four Rhipicephalus sanguineus sensu lato for A. marginale. According to the reviewed literature, this is the first report of A. marginale and T. annulata detection in I. ventalloi. Furthermore, the amplification of A. marginale DNA in several tick species suggests a broad range for this agent in Portugal that might include other uncommon species as R. sanguineus s.l. This work provides new data towards a better understanding of tick-pathogen associations and also contributes to the surveillance of tick-borne agents in geographic areas with limited information.