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  • Previna as doenças de origem alimentar
    Publication . Viegas, Silvia; Santos, Pedro
    O Instituto Ricardo Jorge dá a conhecer as "Cinco Chaves Para Uma Alimentação Mais Segura", através de conselhos simples, que devem ser seguidos.
    2015-09-09Artigo científico Acesso aberto Ver mais
  • Translational control of the human erythropoietin via an upstream open reading frame in cardiac and muscle tissue
    Publication . Onofre, Claudia; Barbosa, Cristina; Romão, Luísa
    Beyond its well-known hematopoietic action, erythropoietin (EPO) has diverse cellular effects in non-hematopoietic tissues. For example, in cases of tissue injury, such as cardiac ischemia or acute myocardial infarct, EPO expression increases locally, providing a cardioprotective effect. Cellular stress activates an integrated stress response, which includes rapid changes in global and gene-specific translation. Translational regulation of specific transcripts mostly occurs at translation initiation and is mediated via different cis-acting elements, including upstream open reading frames (uORFs). The human EPO 5’ untranslated region (5’UTR) has one uORF with 14 codons that is conserved among different species, indicating its potential regulatory role. To test whether EPO expression is translationally regulated in response to ischemia in cardiac tissue, reporter constructs containing the normal or mutant EPO 5’UTR fused to the Firefly luciferase cistron were expressed in H9c2 (heart/myocardium myoblasts) and C2C12 (muscle myoblasts) cell lines. Luminometry assays revealed that the EPO uORF represses translation of the main ORF at about 60-70%, in both cell lines. Under chemical ischemia, EPO uORF-mediated translation repression is specifically released in muscle cells. In response to hypoxia, translational derepression occurs in both cell lines. Although the eIF2-alpha phosphorylation occurs in both conditions, thapsigargin treatment does not affect EPO translation. We are currently exploring additional mechanisms through which EPO cardioprotection effects are regulated at the translational level.
    2015-09-09Documento de conferência Acesso restrito Ver mais
  • Effectiveness of pneumococcal vaccine against community-acquired pneumonia in elderly: feasibility study
    Publication . Rodrigues, Ana Paula; Machado, Ausenda; Nunes, Baltazar; Gonçalves, Paulo; Simões, Maria João
    PneumoPAC 1. Descriptive study 2. Primary Objective To estimate the etiological fraction of Streptococcus pneumoniae CAP and CAP caused by PCV13 serotypes within all CAP cases a>ended in primary health care (Portugal, 2016) 3. Secondary Objective To evaluate the feasibility of conducting a study of vaccine effectiveness against CAP caused by PCV13 serotypes a>ended in primary care seGng using an indirect cohort design 4. Study population Community-dwelling individuals with 65 or more years of age in the catchment area of the participating GP 5. Outcomes of interest Streptoccocus pneumoniae CAP, PCV13 serotypes CAP
    2015-09-09Documento de conferência Acesso restrito Ver mais