Browsing by Author "Vasconcelos, Miguel"
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- Biomarcador de alcoolismo crónico – transferrina deficiente em carbohidratos (CDT): parâmetro bioquímico de diagnóstico e monitorização para utilização no sistema de saúdePublication . Gomes, Filomena; Boto, Paulo; Aguiar, Pedro; Vasconcelos, Miguel; Silva, Ana Vieira daNo âmbito da saúde pública torna-se importante compreender a problemática do diagnóstico e monitorização dos doentes com síndrome de dependência do álcool, pelos problemas de saúde e sociais relacionados com o consumo excessivo, mas também pelos custos imputados ao sistema de saúde. Existem vários biomarcadores específicos do alcoolismo, mas na sua maioria ainda não estão devidamente estandardizados, têm limitações de acesso à amostra biológica, ou são tecnicamente dispendiosos para uso no referido sistema. Tendo em consideração os resultados de uma revisão sistemática da literatura sobre o biomarcador especifico transferrina deficiente em carbohidratos (CDT), realizado no âmbito do Curso de Mestrado em Gestão da Saúde na Escola Nacional de Saúde Pública em 2019, pode concluir-se que a utilização deste parâmetro bioquímico é efetivo no diagnóstico e monitorização dos doentes com síndrome de dependência do álcool.
- Biomarker of Chronic Alcohol Abuse – Carbohydrate-deficient Transferrin (CDT): Methodology VerificationPublication . Gomes, Filomena; Costa, Alcina; Fernandes, Lília; Silva, Ana Maria V. da; Vasconcelos, Miguel; Miranda, ArmandinaIntroduction: Transferrin is a glycoprotein synthesized in hepatocytes that can appear with different isomorphic forms in the plasma, acquiring different levels of sialization (1,2). In a healthy person, penta, tetra and trisial isoforms are detectible in plasma. However, in an alcohol abuse and/or dependence, asialo, monosialo and disialotransferrin isoforms are also present called carbohydrate-deficient transferrin (CDT) (3) . This is considered a specific biomarker of alcohol abusive and/or dependence, being useful in the diagnosis and monitoring of this pathology (4) . Aim: Verify compliance with the requirements of the manufacturer of the capillary electrophoresis method in laboratory practice and its suitability in determining the CDT. Materials and methods: The MiniCap System (Sebia) was used with calibrators traceable to the IFCC international reference procedure and normal and pathological internal control samples. Repeatability and intermediate precision tests were performed on control samples. From participation in External Quality Assessment (EQA) program (5 rounds - 2 samples each), Bias%, Deviation Index (DI) and Total Laboratory Error (TELab) were obtained. The Measurement Uncertainty was calculated by the Top Down Method (combined and expanded with a factor of 1.96), using the internal (CV%) and external (Bias%) quality control results. Results: In the repeatability tests, normal control samples (n=22, mean = 1.4%) were obtained, CV = 5.7%; for the pathological sample (n=24, mean = 5.4%), CV = 2.2%. In intermediate precision tests for the normal control sample, (n= 12, mean = 1.4%), CV = 6.7%; for the pathological sample (n=12, mean = 5.3%), CV = 4.9%. In samples from EQA program, mean Bias = -1.0% and TELab = 11.5. In the evaluation of the method by DI, 1 satisfactory, 7 good and 2 excellent results were obtained. The obtained Expanded Uncertainty (1.3% ± 0.3) is consistent with that indicated by the manufacturer. Conclusion: The TELab obtained meets Westgard`s desirable specifications (5) , being considered an appropriate methodology for use in laboratory practise for diagnosis. However, it is considered important to monitor the method with Internal Control samples, and participate in EQA programs, as well as periodic evaluation of Quality Indicators.