Browsing by Author "Seixas, Elsa"
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- Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helicobacter pylori infectionPublication . Santos, António Mário; Oleastro, Mónica; Lopes, Teresa; Pereira, Teresa; Seixas, Elsa; Chaves, Paula; Machado, Jorge; Guerreiro, António SousaAccumulated evidence in humans and animals shows that H. pylori up-regulate the expression of cyclooxygenase (COX)-2 both at mRNA and protein levels which might be one of the mechanisms leading to several gastric diseases. Aim: To study the expression of COX-2 on mice gastric mucosa during long-term treatment with two nutraceuticals: curcumin and synbiotic 2000 on H. pylori experimental chronic infection. Materials and Methods: We infected 45 C57BL/6 mice with SS1 – H. pylori strain. After infection confirmation by 13C-urea breath test mice where then treated with either PBS, curcumin (10 mg/mouse) or Synbiotic 2000 (50 mg/ mouse), three times per week. Five mice from each treatment group were euthanized at week 6, 18 and 27. Gastric samples were removed for COX-2 immunohistochemistry analysis. Results: All the 45 mice were Hp positive by 13C-urea breath test and immunohistochemistry. In the PBS group the production of COX-2 was significantly up-regulated at week 6 (area of positive immunostaining 393–544 · 103 pixels), 18 (area of positive immunostaining 242–614 · 103 pixels) and 27 week (area of positive immunostaining 129–175 · 103 pixels). The treatment with either curcumin or synbiotic significantly decreased the expression of COX-2 at all time points. Conclusions: These results suggest the therapeutic usefulness of both nutraceuticals on COX-2 inhibition during chronic experimental mice H. pylori infection. The supplementation of diet in humans with curcumin or Synbiotic 2000 may be a novel therapeutic approach against gastric inflammation induced by Hp infection.
- Nutraceuticals: a new therapeutic approach againstPublication . Santos, António Mário; Oleastro, Mónica; Vale de Gato, Inês; Lopes, Teresa; Seixas, Elsa; Machado, Jorge; Guerreiro, António SousaBackground and Aim: H. pylori induces severe gastric chronic inflammation and is the cause of gastritis, peptic ulcer and a major risk factor for gastric cancer. The aim of the study was to investigate the anti-inflammatory effect of two nutraceuticals in Hp-infected mucosa. Materials and Methods: Eighteen C57BL/6 mice were inoculated with Hp SS1 by gavage three times with 3 · 109 viable cells. Mice were then treated with either PBS, curcumin (10 mg/mouse) or Symbiotic 2000 (50 mg/mouse), three times per week. Half of the infected and three non-infected mice were euthanized at week 6, the remaining at week 18. Gastric samples were removed for immunohistochemistry and PCR array (inflammatory response and immunity pathway) analysis (Sabiosciences, Qiagen). Results: All the 18 mice were Hp positive by immunohistochemistry. The production of the chemokines CCL2, CCL5, CCL20, CCL25, CXCL1 and CXCL11 was significantly up-regulated at both week 6 (range of fold-change 4.3–718) and week 18 (range of fold-change 16–1192). Similarly, the expression of the proinflammatory cytokines IL-1b, IL6, IL9, IL10, IL23, TNFa and INFc was significantly augmented (range of fold-change 1338–8251). The treatment with either curcumin or symbiotic drastically decreased the expression of all these mediators, restoring their levels to those similar to the non-infected mice. Conclusions: The present study confirmed that Hp infection induces a strong inflammatory response. Curcumin and Symbiotic treatments exerted a significant anti-inflammatory effect in Hp-infected mucosa. The supplementation of diet with these nutraceuticals may be a novel clinical approach against gastric inflammation induced by Hp infection.