Browsing by Author "Kurtenbach, Klaus"
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- Plasmid profile analysis of Portuguese Borrelia lusitaniae strainsPublication . Vitorino, Liliana; Margos, Gabriele; Zé-Zé, Líbia; Kurtenbach, Klaus; Collares-Pereira, MargaridaPlasmid profilesof2Portuguese Borrelia lusitaniae strains, oneisolatedfromahumanpatientandthe other onefroman Ixodes ricinus tick, wereobtainedbypulsed-fieldgelelectrophoresistoevaluatethe plasmid diversityineachstrain.Overall,amaximumof6plasmidsweredetectedthatrangedfrom 19 kbto76kb,revealingcompletelydifferentplasmidprofilesfromthosepreviouslydescribedforother genospecies of B. burgdorferi sensu lato,thecausativeagentsofLymeborreliosis.Theplasmidlocationof the ospA gene wasinvestigatedbyhybridization,allowingitsallocationtotheplasmidof70kbinstead of the54kblinearplasmiddescribedfor B. burgdorferi sensu strictostrains.