Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

Vitorino, Liliana; Margos, Gabriele; Zé-Zé, Líbia; Kurtenbach, Klaus; Collares-Pereira, Margarida

http://hdl.handle.net/10400.18/183

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Authors

Collares-Pereira, Margarida

Abstract(s)

Plasmid profilesof2Portuguese Borrelia lusitaniae strains, oneisolatedfromahumanpatientandthe other onefroman Ixodes ricinus tick, wereobtainedbypulsed-fieldgelelectrophoresistoevaluatethe plasmid diversityineachstrain.Overall,amaximumof6plasmidsweredetectedthatrangedfrom 19 kbto76kb,revealingcompletelydifferentplasmidprofilesfromthosepreviouslydescribedforother genospecies of B. burgdorferi sensu lato,thecausativeagentsofLymeborreliosis.Theplasmidlocationof the ospA gene wasinvestigatedbyhybridization,allowingitsallocationtotheplasmidof70kbinstead of the54kblinearplasmiddescribedfor B. burgdorferi sensu strictostrains.