Percorrer por autor "Good, Christian R."
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- Endotoxin as a Marker for Water QualityPublication . Sattar, Anas A.; Good, Christian R.; Saletes, Margaux; Brandão, João; Jackson, Simon K.Background: Water quality testing is vital to protect human health. Current testing relies mainly on culture-based detection of faecal indicator organisms such as Escherichia coli (E.coli). However, bacterial cultures are a slow process, taking 24-48 h and requiring specialised laboratories and trained personnel. Access to such laboratories is often sparse in developing countries and there are many fatalities deriving from poor water quality. Endotoxin is a molecular component of Gram-negative bacterial cell walls and can be used to detect their presence in drinking water. Method: The current study used a novel assay (BacterisK) to rapidly detect endotoxin in various water samples and correlate the results with E. coli content measured by culture methods. The data generated by the BacterisK assay are presented as an 'endotoxin risk' (ER). Results: The ER values correlate with E. coli and thus endotoxin can be used as a marker of faecal contamination in water. Moreover, the BacterisK assay provides data in near real-time and can be used in situ allowing water quality testing at different spatial and temporal locations. Conclusion: We suggest that BacterisK can be used as a convenient risk assessment tool to assess water quality where results are required quickly or access to laboratories is lacking.
- A new dimension in rapid water quality assessmentPublication . Good, Christian R.; White, Alistair; Brandão, João; Jackson, Simon K.Microbial pollution of bathing waters from various sources, such as sewage discharge and agricultural runoff, poses a substantial risk to public health. Therefore, monitoring bathing water quality is vital to mitigate the health risks associated with the recreational use of bathing waters Conventional testing for bacterial contamination relies on culture based methods to enumerate faecal indicator bacteria ( which often takes over 48 hours and water quality is thus retrospective with potential impacts on human health. To address this issue, Molendotech have developed a novel rapid water quality kit Bacterisk that provides near real time assessment of bacterial contamination of water which can be conducted by non specialist staff in situ.